FastQCFastQC Report
Sat 23 Sept 2023
SRR18915705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18915705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136616394
Total Bases20.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT12339720.9032385966796928Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC10295680.7536196570962047No Hit
GTTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT6828430.4998250795581678Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC5762520.4218029645841772No Hit
ATTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT4992450.3654356445683964Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT4871950.3566153268545501Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT4277260.31308541198942785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC4124190.30188104657483494No Hit
TTTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT4115950.3012778978780541No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC4041010.29579246543427284No Hit
ACGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC4025290.2946417982603171No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC3852680.28200715062059095No Hit
CTTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC3531040.2584638561020722No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT3109550.22761177549452816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC3104210.2272209000041386No Hit
GCTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT2973070.2176217592158083Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC2610800.19110444387808978No Hit
GCTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC2498980.18291948183026993No Hit
TATCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT2442860.178811629298311Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGACTGCATAATTTGTGGTAGTGGGGGATCGGAAGAGCACACGTCTG2433370.17811698352980976Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT2343340.17152699843621988No Hit
TGTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT2247260.1644941675155033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC2073220.151754847225729No Hit
TTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA2032540.1487771665236604No Hit
TATCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC2000760.14645094497224104No Hit
GATCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1964440.14379240605633317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1934920.1416316112105843No Hit
ATGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1836260.1344099303338368No Hit
CTGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1831000.1340249106560374No Hit
CTTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1783130.13052093879743304No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1777860.13013518714305986No Hit
AATCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1745100.1277372318874117Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1726350.12636477581160574Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1703720.12470831282517968No Hit
GATCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1639280.11999145578384977No Hit
TTTCGACTGCATAATTTGTGGTAGTGGGGATCGGAAGAGCACACGTCTGA1610930.11791630219723118Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1570300.11494228137803139Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1475080.10797240044265843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC1452750.10633789675344528No Hit
AATCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1441960.10554809403035481No Hit
GGTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1420840.10400215950656698No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1403330.10272046852590766No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1400110.1024847720691559Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC1394080.10204339019517672No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)