FastQCFastQC Report
Sat 23 Sept 2023
SRR19142907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19142907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815068
Total Bases193.1 Mbp
Sequences flagged as poor quality0
Sequence length5-105
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCC37024613.152293301618293TruSeq Adapter, Index 16 (100% over 30bp)
TGGTCATTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1011073.5916361523060902No Hit
TGGTCATTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC384321.3652245700636716No Hit
TGGTCATTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC284141.0093539481106675No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCCCG224950.799092597407949TruSeq Adapter, Index 6 (96% over 31bp)
TGGTCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179550.6378176299826505No Hit
TACAAGTTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC112840.40084289260508094No Hit
TGGTCATTTTTTTTTTTAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATC112730.40045213827872006No Hit
TGGTCATTTTTTTTTTTGCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCC112360.39913778281732454No Hit
TGGTCATTTTTTTTTTTCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCC103670.36826819103481695No Hit
TGGTCATTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92650.3291217121575749No Hit
TGGTCATTTTTTTTTTTGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCC89840.31913971527508395No Hit
TGGTCATTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84150.29892705966605426No Hit
TGGTCATTTTTTTTTTTGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCCC84030.29850078221911513No Hit
GGAGAGCACACGTCTGAACTCCAGTCACCC72880.2588925027743557TruSeq Adapter, Index 16 (96% over 30bp)
TGGTCATTTTTTTTTTTGGGGGTCGGGAGGAACGGGGGGCGGGAAAGATC71930.25551780631942106No Hit
TGGTCATTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCC64270.22830709595647422No Hit
GAAGAGCACCCGTCTGAACTCCAGTCACCC61360.21796986786820072TruSeq Adapter, Index 16 (96% over 30bp)
TGGTCATTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCCC56150.1994623220469275No Hit
TGGTCATTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55740.19800587410321882No Hit
TGGTCATTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCC54340.1930326372222625No Hit
TACAAGTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54120.19225112856954077No Hit
TACAAGTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC52160.1852885969362019No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTACGATATCTCGTATGCCGTCT47780.1697294701229242TruSeq Adapter, Index 7 (96% over 30bp)
GATCTGTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47470.16862825338499818No Hit
GAAGAGCCCACGTCTGAACTCCAGTCACCC47470.16862825338499818TruSeq Adapter, Index 16 (96% over 30bp)
TGGTCATTTTTTTTTTTGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGC46510.16521803380948524No Hit
TGGTCATTTTTTTTTTTGTCGGCGCGCCCCCGCGGGGGAGACCCCCCCC43950.1561241149414508No Hit
TGGTCATTTTTTTTTTTGGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC43200.15345988089808132No Hit
TGGTCATTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41810.14852216713770325No Hit
GGGTGACTGGAGCACACGTCTGAACTCCAGTCACCC39120.1389664477021514TruSeq Adapter, Index 16 (100% over 27bp)
TGGTCATTTTTTTTTTTGCCCCGGGGGCGGACCCGGCGGGGGGGACCGGC38490.13672849110572108No Hit
GAAGAGCACACGTCTGAACTCCAGTCACC37080.13171973110418647TruSeq Adapter, Index 2 (100% over 29bp)
TGGTCATTTTTTTTTTTCCC36800.1307250837279952No Hit
TGTTCATTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36220.12866474273445616No Hit
GGGAGAGCACACGTCTGAACTCCAGTCACCC33220.11800780656097828TruSeq Adapter, Index 16 (96% over 31bp)
TGGTCATTTTTTTTTTTGCCCCGCGGCGGGCCGCCGCCGGCCCCCC33110.11761705223461742No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCCC32890.11683554358189571TruSeq Adapter, Index 6 (96% over 31bp)
TGGTCATTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC31660.11246619975076978No Hit
TGGTCATTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC30390.10795476343733082No Hit
TGGTCATTTTTTTTTTTCACCGGCGCGCCCCCGCGGGGGAGACCCCCCCC30330.10774162471386126No Hit
CAAGAGCACACGTCTGAACTCCAGTCACCC29920.10628517677015262TruSeq Adapter, Index 16 (96% over 30bp)
TGGTCATTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC28690.10191583293902669No Hit
TGGTCATTTTTTTTTTTCCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCC28200.10017520003069197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)