FastQCFastQC Report
Sat 23 Sept 2023
SRR19142915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19142915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62724682
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length20-101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGCAGCTATCTCGTATGCCGTCT38143266.081060721838335TruSeq Adapter, Index 5 (96% over 32bp)
GATCTGTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27172074.331958191513829No Hit
GATCTGTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC16142372.5735275947672402No Hit
GATCTGTTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC12652102.0170847577991706No Hit
GATCTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11528091.8378873566867984No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCC6243180.9953306738167281TruSeq Adapter, Index 16 (100% over 30bp)
GATCTGTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720310.5931173951587351No Hit
NNNNNNNNNNNNNNNNNNNN3248610.5179157384966894No Hit
GATCTGTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2715820.4329746940765678No Hit
GATCTGTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCC2390530.3811147260977744No Hit
GATCTGTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2277490.3630931122137853No Hit
GATCTGTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2057480.3280176055735125No Hit
GATCTGTTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC1924380.3067978885887377No Hit
GATCTGTTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCC1821870.2904550396923495No Hit
GATCTGTTTTTTTTTTTCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCC1692060.26975983712440343No Hit
GATCTGTTTTTTTTTTTGTCGGCGCGCCCCCGCGGGGGAGACCCCCCCC1575590.25119138906116734No Hit
GATCTGTTTTTTTTTTTGGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC1515200.24156360011518274No Hit
GATCTGTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCCC1415630.2256894662295777No Hit
GATCTGTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC1315480.20972286475681137No Hit
GATCTGTTTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCC1142250.18210534730172087No Hit
GATCTGTTTTTTTTTTTGCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCC1117200.17811170409759908No Hit
GATCTGTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC1068600.17036355800097958No Hit
GATCTGTTTTTTTTTTTCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC1006900.16052691984950995No Hit
GATCTGTTTTTTTTTTTCCC779800.12432107666962744No Hit
GATCTGTTTTTTTTTTTAGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC719320.11467893930494537No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)