FastQCFastQC Report
Sat 23 Sept 2023
SRR19142916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19142916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218141929
Total Bases15.3 Gbp
Sequences flagged as poor quality0
Sequence length10-105
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGCTACGATCTCGTATGCCGTCT2365030310.841704347448031TruSeq Adapter, Index 6 (96% over 32bp)
GATCTGTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185914778.522651782363216No Hit
GATCTGTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC81072813.716516598695705No Hit
GATCTGTTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC46089482.112820777338959No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCC27057781.2403750220802348TruSeq Adapter, Index 16 (100% over 30bp)
GATCTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26801851.2286427521230914No Hit
GATCTGTTTTTTTTTTTGCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCC25449531.1666500849545527No Hit
GATCTGTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23286051.0674724527626234No Hit
GATCTGTTTTTTTTTTTGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCC21171190.9705236447230647No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGCTACGAACTCGTATGCCGTCT20650080.9466350689509122TruSeq Adapter, Index 6 (96% over 32bp)
GATCTGTTTTTTTTTTTCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCC20051510.9191955939841351No Hit
NNNNNNNNNNNNNNNNNNNN16286580.7466047483241977No Hit
GATCTGTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13239890.6069392555889611No Hit
GATCTGTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12685000.5815021467055974No Hit
GATCTGTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCC11443670.5245974514143038No Hit
GATCTGTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9695930.4444780535520065No Hit
GATCTGTTTTTTTTTTTGTCGGCGCGCCCCCGCGGGGGAGACCCCCCCC8214240.3765548437962149No Hit
GATCTGTTTTTTTTTTTCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCC8081140.3704533116143848No Hit
GATCTGTTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC7922850.36319702664772896No Hit
GATCTGTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCCC7812220.35812555778765487No Hit
GATCTGTTTTTTTTTTTGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCCC7603670.3485652682570713No Hit
GATCTGTTTTTTTTTTTAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGC6561980.30081241282137927No Hit
GATCTGTTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCC6048390.27726856674124306No Hit
GATCTGTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC6021330.27602808995055694No Hit
GATCTGTTTTTTTTTTTGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCC5403410.24770157781083893No Hit
GATCTGTTTTTTTTTTTCCC5171120.23705300598125728No Hit
GATCTGTTTTTTTTTTTGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCC4687260.21487203406915872No Hit
GATCTGTTTTTTTTTTTGGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC4673240.21422933323377735No Hit
GATCTGTTTTTTTTTTTCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC4486300.20565968315059685No Hit
GATCTGTTTTTTTTTTTAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCC3942790.18074425297669391No Hit
GATCTGTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC3662620.16790077986337051No Hit
GATCTGTTTTTTTTTTTGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGA3584230.16430724787438733No Hit
GATCTGTTTTTTTTTTTTCGCCGGCGCGCCCCCGCGGGGGAGACCCCCCC3440670.1577262113603112No Hit
GATCTGTTTTTTTTTTTAGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC3058450.14020459129615565No Hit
GATCTGTTTTTTTTTTTTGCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGC3035980.13917452797439964No Hit
GATCTGTTTTTTTTTTTGGTCGCGCCGTGGGAGGGGTGGCCCCGCCCCCC2963150.13583587591727952No Hit
GATCTGTTTTTTTTTTTGCGGCCGGGCGCTTGGCGCCAGAAGCGAGCC2886350.13231523225413488No Hit
GATCTGTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2833590.12989662340429747No Hit
GATCTGTTTTTTTTTTTGGGCAGATCGCAACTCCCAGGCATCC2751310.12612476714643886No Hit
TACAAGTTTTTTTTTTTGCCGGCGCGCCCCCGCGGGGGAGACCCCCCCC2253870.10332126475327906No Hit
GATCTGTTTTTTTTTTTGGGGGGGGCGGCGGCGCCTCGTCCAGCCGCGGC2212960.10144588021865342No Hit
GATCTGTTTTTTTTTTTGGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGG2181890.10002157815336822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)