FastQCFastQC Report
Sat 23 Sept 2023
SRR19217721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19217721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29739675
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACCAGTGGATCTCGT1706800.5739134674471056TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACCAGTGGATCGCGT1035770.34827885644345474TruSeq Adapter, Index 4 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)