FastQCFastQC Report
Sat 23 Sept 2023
SRR19217725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19217725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32636233
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGGCTACATCTCGT3514111.0767511066611148TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGGCTACATCGCGT2128650.6522352012868642TruSeq Adapter, Index 2 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)