FastQCFastQC Report
Sat 23 Sept 2023
SRR19217727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19217727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26575655
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGCAATAATCTCGT728770.27422466163110565TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGCAATAATCGCGT311780.11731789865574338TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)