FastQCFastQC Report
Sat 23 Sept 2023
SRR19217731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19217731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35853451
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCACCGCTATCTCGT892160.24883518186296769TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCACCGCTATCGCGT415610.11591910636440549TruSeq Adapter, Index 18 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)