FastQCFastQC Report
Sat 23 Sept 2023
SRR19240764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19240764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98728691
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length10-134
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA69346597.023955174286672No Hit
AAGAGGTGCACAATCGACCGATCCTG37218463.7697714436424565No Hit
CACGTTCTAGCATTCAAGGTCCC21581992.1859896835865067No Hit
ATATAGATGGATACGAATAAGGCGTC18932121.9175905006174954No Hit
TTGTGGCGTCGCTGAACCATAG18233131.846791425604944No Hit
TGTGGCGTCGCTGAACCATAG16355271.6565873439970962No Hit
ACGTTCTAGCATTCAAGGTCCC12764751.2929119054156204No Hit
AGAGGTGCACAATCGACCGATCCTGA12569301.2731152284800373No Hit
TAGATGGATACGAATAAGGCGTC12447711.2607996595437492No Hit
ATAGATGGATACGAATAAGGCGTC12166331.2322993323187077No Hit
TATAGATGGATACGAATAAGGCGTC10282471.0414875246345563No Hit
CACGTTCTAGCATTCAAGGTCC9803570.9929808549776072No Hit
GTGCCCGAGTTGTAATTTG9259860.9379097308197878No Hit
TCGGTGCCCGAGTTGTAATTTG9192170.9310535678022915No Hit
AGATGGATACGAATAAGGCGTC8703950.8816028969734846No Hit
AGAGGTGCACAATCGACCGATCCTG8618200.8729174784663153No Hit
TTACTTATTCAATGAAGCGGAG8588720.869931517678078No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA8299240.840610760250027No Hit
AATATAGATGGATACGAATAAGGCGTC8108780.8213195088345696No Hit
GTGCCCGAGTTGTAATTT7709510.7808783770869605No Hit
TGCCCGAGTTGTAATTT7595190.7692991695797932No Hit
TTGTGGCGTCGCTGAACCATA7194020.7286655912413545No Hit
TGCCCGAGTTGTAATTTG7180590.7273052977072288No Hit
ATGATTAATAGGGACGGTCGGGGGC7138070.7229985455798255No Hit
TCGGTGCCCGAGTTGTAATTT6817820.6905611662571319No Hit
CACGTTCTAGCATTCAAGGTCCCA6713280.6799725522543391No Hit
TGTGGCGTCGCTGAACCATA6672260.6758177316460116No Hit
CAAGAGGTGCACAATCGACCGATCCTGA6531610.6615716195406662No Hit
AAATATAGATGGATACGAATAAGGCGTC5735350.5809202919544432No Hit
GTGGCGTCGCTGAACCATAG5496010.5566780987707007No Hit
ATATAGATGGATACGAATAAGGCGTCC5471610.5542066793937337No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT5336990.5405713319950732No Hit
ACGTTCTAGCATTCAAGGTCC5241080.5308568306653635No Hit
AAGAGGTGCACAATCGACCGATCCT5072040.5137351613423092No Hit
TTGTGGCGTCGCTGAACCATAGC5006860.5071332304000667No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT4603490.46627681916698355No Hit
ATGATTAATAGGGACGGTCGGGGGCA4445860.4503108422656997No Hit
ACGTTCTAGCATTCAAGGTCCCA4287550.43427598974243464No Hit
GTGGCGTCGCTGAACCATA3660590.37077266627590555No Hit
TTATAGATGGATACGAATAAGGCGTC3613570.3660101195912746No Hit
TGTGGCGTCGCTGAACCATAGC3589500.36357212514850423No Hit
CAAGAGGTGCACAATCGACCGATCCTG3441290.34856027818701657No Hit
ACTATGCCGACTAGGGATCGGGTGGTG3235770.3277436343200377No Hit
ATAGATGGATACGAATAAGGCGTCC3206940.3248235105233999No Hit
ATAAATCATTTGTATACGACTTAGAT3157760.3198421824512998No Hit
TAGATGGATACGAATAAGGCGTCC3078710.3118353913960026No Hit
TTTGTGGCGTCGCTGAACCATAG2989260.30277520847511286No Hit
TACTTATTCAATGAAGCGGAG2929510.29672326963192497No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT2845680.2882323234691727No Hit
TCACGTTCTAGCATTCAAGGTCCC2777130.2812890530474065No Hit
TATAGATGGATACGAATAAGGCGTCC2744910.27802556401765727No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT2729220.27643636032812385No Hit
CAATATAGATGGATACGAATAAGGCGTC2714230.27491805801415925No Hit
ATGTATTTATTAGATA2627900.2661738926529472No Hit
ATGATTAATAGGGACGGTCGGGGGCAT2522550.25550323562985355No Hit
TTTCGGTGCCCGAGTTGTAATTTG2462000.24937026664315848No Hit
AATATAGATGGATACGAATAAGGCGTCC2392880.24236926224414337No Hit
AGTCAAGGATGCTGGCA2367510.2397995938181739No Hit
AAAGAGGTGCACAATCGACCGATCCTGA2194680.22229404419025467No Hit
ATGTATTTATTAGAT2189680.22178760579333517No Hit
TTTACTTATTCAATGAAGCGGAG2166030.21939215217590596No Hit
AGATGGATACGAATAAGGCGTCC2043820.20701378487839972No Hit
ATTAAGCCTTTGTTGTCTGATTTGT1996910.20226237983850104No Hit
AGGTGCACAATCGACCGATCCTGA1959260.19844889870969726No Hit
ATGGATACGAATAAGGCGTC1957250.1982453104741356No Hit
AATCGACCGATCCTGA1940860.19658520540903351No Hit
GATGGATACGAATAAGGCGTC1854910.18787952936598742No Hit
TCGAAGAGTCGAGTTGTT1837110.18607660867295406No Hit
TTGTGGCGTCGCTGAACCATAGCA1835530.1859165741395275No Hit
TGGCGTCGCTGAACCATAG1832040.18556308013847767No Hit
TCAATATAGATGGATACGAATAAGGCGTC1822800.18462718198097045No Hit
TTTCTTTACTTATTCAATGAAGCGGAG1815580.1838958849358187No Hit
AAGAGGTGCACAATCGACCGA1809980.18332867393126887No Hit
CTATGCCGACTAGGGATCGGGTGGTG1769030.17918094346049823No Hit
ACAATCGACCGATCCTGA1728490.17507474093827496No Hit
TAAGAGGTGCACAATCGACCGATCCTGA1709080.1731087470814335No Hit
GTGCCCGAGTTGTAATT1678110.16997186765091418No Hit
TGCCCGAGTTGTAATT1632700.16537239413009133No Hit
GAGGTGCACAATCGACCGATCCTG1613760.16345400548256028No Hit
AAATATAGATGGATACGAATAAGGCGTCC1612670.16334360191203182No Hit
GAGGTGCACAATCGACCGATCCTGA1609070.1629789662662498No Hit
TTTCGGTGCCCGAGTTGTAATTT1597060.16176250123684918No Hit
CTATGCCGACTAGGGATCGGGTGGTGT1547610.1567538254913154No Hit
CACGTTCTAGCATTCAAGGTC1532590.15523248454696922No Hit
TATATAGATGGATACGAATAAGGCGTC1482950.1502045641423525No Hit
ATATAGATGGATACGAATAAGGCGT1454370.14730976226556067No Hit
AAGAGGTGCACAATCGACCGATCC1428960.1447360423324158No Hit
TTCGGTGCCCGAGTTGTAATTTG1397200.1415191456351832No Hit
CGGTGCCCGAGTTGTAATTTG1375060.13927663641362367No Hit
AGAGGTGCACAATCGACCGATCCT1371710.13893732268768763No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT1356020.13734811899815424No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1343290.13605872683959722No Hit
ATACGAATAAGGCGTC1320560.1337564578872012No Hit
ACGTTCTAGCATTCAAGGTC1305800.13226145173949486No Hit
TTGCTCGAATATATTAGCATGGAATAATAGA1287430.13040079706921262No Hit
TCGAAGAGTCGAGTTGTTT1282970.12994905401916046No Hit
AAAGAGGTGCACAATCGACCGATCCTG1236800.12527260186200584No Hit
CGGTGCCCGAGTTGTAATTT1231020.12468715907516692No Hit
TTGCCCGAGTTGTAATTTG1228430.12442482398556261No Hit
TTCTTTACTTATTCAATGAAGCGGAG1196810.12122210756344373No Hit
TTTGTGGCGTCGCTGAACCATA1188950.1204259864034863No Hit
TGTGGCGTCGCTGAACCATAGCA1174670.11897959834188422No Hit
CCACGTTCTAGCATTCAAGGTCCC1163540.11785226647034143No Hit
TCACGTTCTAGCATTCAAGGTCC1136330.11509622871430555No Hit
TTGCCCGAGTTGTAATTT1126520.11410259657954949No Hit
TGGCGTCGCTGAACCATA1125860.11403574671115613No Hit
TAGATGGATACGAATAAGGCGT1109130.1123412038350635No Hit
AGGTGCACAATCGACCGATCCTG1102790.1116990399477696No Hit
TTGTGGCGTCGCTGAACCAT1066420.1080152070485772No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA1066150.10798785937514355No Hit
ATCGACCGATCCTGA1061220.10748851111578094No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGA1060250.10739026206677854No Hit
AGAGGTGCACAATCGACCGATC1039440.10528246545879962No Hit
TCACGTTCTAGCATTCAAGGTCCCA1031020.1044296231983872No Hit
TTCGGTGCCCGAGTTGTAATTT1007280.10202505368981343No Hit
TCCATCTAAAGCTAAATA1006080.10190350847455276No Hit
TGTGGCGTCGCTGAACCAT992230.10050067411508576No Hit
AAGAGGTGCACAATCGACCGATC990020.10027682834364733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)