FastQCFastQC Report
Sat 23 Sept 2023
SRR19240765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19240765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94681694
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length10-134
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA71536537.555476352165816No Hit
AAGAGGTGCACAATCGACCGATCCTG35587373.758632582133564No Hit
ACGTTCTAGCATTCAAGGTCCC20507722.165964626699645No Hit
CACGTTCTAGCATTCAAGGTCCC19983212.1105674345032313No Hit
ATATAGATGGATACGAATAAGGCGTC19594262.069487687873434No Hit
AGAGGTGCACAATCGACCGATCCTGA13328861.4077547028256592No Hit
ATAGATGGATACGAATAAGGCGTC12782911.3500930813510792No Hit
TTGTGGCGTCGCTGAACCATAG12770121.3487422394449344No Hit
TAGATGGATACGAATAAGGCGTC11987751.2661106380289309No Hit
TATAGATGGATACGAATAAGGCGTC10956361.1571782820024321No Hit
TGTGGCGTCGCTGAACCATAG10612991.1209125599294834No Hit
TCGGTGCCCGAGTTGTAATTTG9814921.0366227710290017No Hit
AATATAGATGGATACGAATAAGGCGTC9419850.9948966481313696No Hit
CACGTTCTAGCATTCAAGGTCC9350110.9875309159550948No Hit
AAATATAGATGGATACGAATAAGGCGTC9244440.976370363631221No Hit
TCGGTGCCCGAGTTGTAATTT9079330.9589319346145202No Hit
ACGTTCTAGCATTCAAGGTCC8696830.9185334178748429No Hit
AGATGGATACGAATAAGGCGTC8422520.8895616083928536No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT8357200.8826627035211263No Hit
GTGCCCGAGTTGTAATTT8258900.8722805487616223No Hit
GTGCCCGAGTTGTAATTTG7998420.8447694229044952No Hit
TTACTTATTCAATGAAGCGGAG7710270.8143358736272717No Hit
AGAGGTGCACAATCGACCGATCCTG7650490.8080220871417869No Hit
TTGTGGCGTCGCTGAACCATA7218420.7623881338667219No Hit
TGCCCGAGTTGTAATTT7082470.7480294976555869No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA6176630.6523573606530529No Hit
CAAGAGGTGCACAATCGACCGATCCTGA5756600.6079950365062121No Hit
TGCCCGAGTTGTAATTTG5739460.6061847604881255No Hit
AAGAGGTGCACAATCGACCGATCCT5697790.6017836985468384No Hit
ATGATTAATAGGGACGGTCGGGGGC5641070.5957931001952711No Hit
TGTGGCGTCGCTGAACCATA5608230.5923246366927064No Hit
ATATAGATGGATACGAATAAGGCGTCC5212400.550518244846781No Hit
ACGTTCTAGCATTCAAGGTCCCA4987430.5267575799816172No Hit
TTGTGGCGTCGCTGAACCATAGC4980610.5260372717877227No Hit
CACGTTCTAGCATTCAAGGTCCCA4658750.4920433721855463No Hit
TTATAGATGGATACGAATAAGGCGTC4464080.4714829035483882No Hit
TGTGGCGTCGCTGAACCATAGC3551620.37511158175940534No Hit
ATGATTAATAGGGACGGTCGGGGGCA3545450.3744599246397091No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT3394260.3584916847812208No Hit
TCACGTTCTAGCATTCAAGGTCCC3282670.3467058795969578No Hit
ATAGATGGATACGAATAAGGCGTCC3161840.3339441729887089No Hit
GTGGCGTCGCTGAACCATAG3069520.32419360811182785No Hit
ATAAATCATTTGTATACGACTTAGAT3035760.32062797693501344No Hit
CAAGAGGTGCACAATCGACCGATCCTG2992730.3160832758230963No Hit
TAGATGGATACGAATAAGGCGTCC2959970.3126232616835098No Hit
TTTCGGTGCCCGAGTTGTAATTTG2914620.3078335290452239No Hit
AAAGAGGTGCACAATCGACCGATCCTGA2848000.30079732202510023No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT2757090.29119567717071054No Hit
TACTTATTCAATGAAGCGGAG2714970.2867470875626708No Hit
ACTATGCCGACTAGGGATCGGGTGGTG2707360.2859433419093664No Hit
TATAGATGGATACGAATAAGGCGTCC2686820.283773967964705No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT2649290.2798101605575414No Hit
TTTCGGTGCCCGAGTTGTAATTT2541900.2684679469296356No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT2537030.2679535919583357No Hit
AATATAGATGGATACGAATAAGGCGTCC2495310.26354724916518707No Hit
TTTGTGGCGTCGCTGAACCATAG2432840.2569493528495593No Hit
AAATATAGATGGATACGAATAAGGCGTCC2310910.24407146750035968No Hit
TAAGAGGTGCACAATCGACCGATCCTGA2209980.23341153993294625No Hit
ATGATTAATAGGGACGGTCGGGGGCAT2162830.22843169662765012No Hit
ATGGATACGAATAAGGCGTC2096470.2214229500372057No Hit
AGTCAAGGATGCTGGCA2081580.21985031235288205No Hit
ACGTTCTAGCATTCAAGGTC2077440.2194130578187585No Hit
ATTAAGCCTTTGTTGTCTGATTTGT2034980.21492855841806127No Hit
AGATGGATACGAATAAGGCGTCC2027180.21410474552768355No Hit
TTTACTTATTCAATGAAGCGGAG2005330.2117970132642536No Hit
TTTCTTTACTTATTCAATGAAGCGGAG1997980.21102072804062844No Hit
GTGGCGTCGCTGAACCATA1982700.2094068997117859No Hit
TCGAAGAGTCGAGTTGTT1972260.20830425784312645No Hit
TATATAGATGGATACGAATAAGGCGTC1966160.2076599939160362No Hit
AGGTGCACAATCGACCGATCCTGA1930550.20389897122035017No Hit
AAGAGGTGCACAATCGACCGATCC1840910.1944314600032399No Hit
ATATAGATGGATACGAATAAGGCGT1770490.1869939082416502No Hit
GTGCCCGAGTTGTAATT1710850.18069490814137737No Hit
TTCGGTGCCCGAGTTGTAATTTG1690900.17858784824868046No Hit
ACAATCGACCGATCCTGA1671800.17657056283762732No Hit
CAATATAGATGGATACGAATAAGGCGTC1638360.17303872911272586No Hit
CGGTGCCCGAGTTGTAATTTG1609180.1699568239664153No Hit
CGGTGCCCGAGTTGTAATTT1588330.16775470874021328No Hit
GAGGTGCACAATCGACCGATCCTGA1575800.1664313272637475No Hit
CACGTTCTAGCATTCAAGGTC1566320.1654300777508269No Hit
AAGAGGTGCACAATCGACCGATC1562450.16502133981675488No Hit
TTGTGGCGTCGCTGAACCATAGCA1553980.16412676351143443No Hit
TTCGGTGCCCGAGTTGTAATTT1550750.16378562048118825No Hit
AATCGACCGATCCTGA1542500.16291427992405796No Hit
AAAGAGGTGCACAATCGACCGATCCTG1490470.15741902547708958No Hit
TTTGTGGCGTCGCTGAACCATA1485360.15687932241685493No Hit
TCGAAGAGTCGAGTTGTTT1473100.1555844575404407No Hit
AGAGGTGCACAATCGACCGATCCT1448330.15296832352830528No Hit
CTATGCCGACTAGGGATCGGGTGGTG1441730.15227125108260103No Hit
GATGGATACGAATAAGGCGTC1425360.1505423001831801No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1418260.14979241921886188No Hit
TCACGTTCTAGCATTCAAGGTCC1413750.14931608638096397No Hit
TGCCCGAGTTGTAATT1357690.1433951952739671No Hit
ATACGAATAAGGCGTC1342180.14175707502656215No Hit
TCAATATAGATGGATACGAATAAGGCGTC1341060.1416387839448669No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT1258110.13287785070681138No Hit
TTCTTTACTTATTCAATGAAGCGGAG1253890.13243214680970958No Hit
TTGCCCGAGTTGTAATTT1252050.13223781146121022No Hit
ATGTATTTATTAGATA1227160.12960900340460746No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGTT1215940.12842398024691024No Hit
TTGCCCGAGTTGTAATTTG1204840.1272516311336804No Hit
TAGATGGATACGAATAAGGCGT1164330.12297308495557759No Hit
TAAGAGGTGCACAATCGACCGATCCTG1160300.12254744829554907No Hit
TGGCGTCGCTGAACCATAG1146220.12106036041138005No Hit
ATAGATGGATACGAATAAGGCGT1134660.11983942746102536No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT1130930.11944547591216524No Hit
TAATATAGATGGATACGAATAAGGCGTC1114500.11771018799051061No Hit
ATGTATTTATTAGAT1114040.11766160415338577No Hit
AGAGGTGCACAATCGACCGATC1107750.11699727298922218No Hit
TTATAGATGGATACGAATAAGGCGTCC1104480.11665190527748691No Hit
TTTTACTTATTCAATGAAGCGGAG1097610.11592631623173114No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT1088230.1149356284225333No Hit
CTATGCCGACTAGGGATCGGGTGGTGT1080480.11411709638401696No Hit
TTCTAGCATTCAAGGTCCC1079510.11401464785790588No Hit
TTTGTGGCGTCGCTGAACCATAGC1060720.11203010372839338No Hit
TTGTGGCGTCGCTGAACCAT1023730.10812332952133281No Hit
TGTGGCGTCGCTGAACCATAGCA1015970.1073037413124442No Hit
CCACGTTCTAGCATTCAAGGTCCC1014820.10718228171963208No Hit
TATAGATGGATACGAATAAGGCGT995080.10509740140475306No Hit
GAGGTGCACAATCGACCGATCCTG969190.10236297631092237No Hit
TCTATGCCGACTAGGGATCGGGTGGTG966240.10205140605110002No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA959870.10137862552395821No Hit
TTGCTCGAATATATTAGCATGGAATAATAGA957390.10111669527163296No Hit
AGGTGCACAATCGACCGATCCTG954080.10076710287840857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)