FastQCFastQC Report
Sat 23 Sept 2023
SRR19240770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19240770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34979062
Total Bases798.6 Mbp
Sequences flagged as poor quality0
Sequence length10-134
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGTCGCTGAACCATA11811993.3768744284795287No Hit
AGATGGATACGAATAAGGCGTC8969182.5641568090076285No Hit
GAGGTGCACAATCGACCGATCCTG8874082.5369691159814405No Hit
AGGGGGCATGCCTGTTTGAGCGTCATT7832292.2391366583815198No Hit
TGCCCGAGTTGTAATTT6793861.9422647754247957No Hit
TAGATGGATACGAATAAGGCGTC6278271.7948651681969061No Hit
ATAAATCATTTGTATACGACTTAGAT4455611.273793448206244No Hit
AGAGGTGCACAATCGACCGATCCTG4205161.2021934721977392No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACG4171121.1924619362291646No Hit
AATACTGCCAGCTGGGACTGAGGA3981961.138383870899683No Hit
GTCAAGGATGCTGGCA3593711.0273888991077005No Hit
TGGCGTCGCTGAACCATA3593491.027326004339396No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT3296650.9424638087779484No Hit
AGGGGGCATGCCTGTTTGAGCGTCAT3268750.9344876086156914No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATT3159700.9033118155083748No Hit
GATGGATACGAATAAGGCGTC3118630.8915705058071597No Hit
ATCTTTGAACGCACATTGCGCCCCT2978880.8516180336682557No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT2493220.7127749737828876No Hit
GTGGCGTCGCTGAACCATAG2025700.5791178734295391No Hit
TCTTTGAACGCACATTGCGCCCCT1964730.5616874460498683No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACG1902440.5438796500603704No Hit
ATGCAGCTCTAAGTGGGTGGT1876710.5365238210218445No Hit
TCGAATCTTTGAACGCACATTGCGCCCCT1824820.5216892322612882No Hit
TGTGGCGTCGCTGAACCATA1813860.5185559292584804No Hit
GCCCGAGTTGTAATTT1791220.5120834858293227No Hit
AGGTGCACAATCGACCGATCCTG1790980.5120148733548087No Hit
AAATACTGCCAGCTGGGACTGAGGA1781200.5092189150183616No Hit
AATACTGCCAGCTGGGACTGAGG1580540.45185316861841524No Hit
TCCATCTAAAGCTAAATATT1546710.4421816685650404No Hit
TCGAAGAGTCGAGTTGTT1474090.421420677318334No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA1455410.416080339718658No Hit
ATGGATACGAATAAGGCGTC1403610.4012714806360445No Hit
GAGGTGCACAATCGACCGATCCT1371420.392068832491849No Hit
AGATGGATACGAATAAGGCGT1370710.3918658539214116No Hit
ATGCCTGTTTGAGCGTCATT1341060.383389354465823No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATA1187670.33953740669203764No Hit
ATAGATGGATACGAATAAGGCGTC1184130.3385253726929556No Hit
TCCATCTAAAGCTAAATATTG1123340.3211464046691704No Hit
TGAGGTGCACAATCGACCGATCCTG1116210.31910804240548246No Hit
TCCATCTAAAGCTAAATA1109080.31706968014179454No Hit
CAGGGGGCATGCCTGTTTGAGCGTCAT1101240.3148283393076693No Hit
TTGATGATTCATAATAACTTT1065640.30465082225475343No Hit
ATGCAGCTCTAAGTGGGTGGTA1054730.3015318135174694No Hit
TGGCGTCGCTGAACCATAG1043600.2983499100118808No Hit
TAGATGGATACGAATAAGGCGT1034140.2956454349747858No Hit
TTGCCCGAGTTGTAATTT1006260.2876749525187382No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT1002700.2866572008134466No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA981370.28055926714101137No Hit
TCTTTGAACGCACATTGCGCCCC979670.2800732621132036No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATT977820.27954437428882456No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAT966950.2764368009639595No Hit
AGGTGCACAATCGACCGATC958160.27392386908488287No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATT925400.2645582663137165No Hit
TGCCCGAGTTGTAATT901400.25769701886231255No Hit
GTGGCGTCGCTGAACCAT855890.24468637838258783No Hit
ATCTTTGAACGCACATTGCGCCCC849280.24279667648034703No Hit
AATGCAGCTCTAAGTGGGTGGT810860.2318129628518912No Hit
GAGGTGCACAATCGACCGATCCTGA810540.2317214795525392No Hit
CGAATCTTTGAACGCACATTGCGCCCCT785320.22451145202235556No Hit
ATCAGTTTTGGTGGCAGGATA774940.22154396249962335No Hit
AGTTTTGGTGGCAGGATA765820.21893668846808986No Hit
ATGCCTGTTTGAGCGTCAT738120.21101766536792782No Hit
AATGCAGCTCTAAGTGGGTGGTA728010.20812736487902392No Hit
AATCGACCGATCCTG711420.20338452757824094No Hit
TCGAAGAGTCGAGTTGTTT704400.2013776126987053No Hit
AGAGGTGCACAATCGACCGATCCT669830.19149455751557887No Hit
AGGTGCACAATCGACCGATCCT668000.19097138739740935No Hit
AAATACTGCCAGCTGGGACTGAGG667640.19086846868563828No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACG662420.18937614736495792No Hit
ACAATCGACCGATCCTG631340.18049083191538984No Hit
TAGGTGCACAATCGACCGATCCTG630770.180327877288419No Hit
CCATCTAAAGCTAAATATT628640.1797189415771069No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATA623580.17827236190610257No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAT620690.17744615335882935No Hit
AATCTTTGAACGCACATTGCGCCCCT597940.17094226254551936No Hit
ATTAAGCCTTTGTTGTCTGATTTGT573710.16401526147270615No Hit
AGGGGGCATGCCTGTTTGAGCGTCA570100.16298321550189082No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACG560700.16029589358342428No Hit
TTTTGGTGGCAGGATA554520.15852912236468777No Hit
GGCCAGCATCAGTTTTGGTGGCAGGATA541560.15482404874092964No Hit
TTGGCGTCGCTGAACCATA521340.14904344776312184No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGT510520.14595016870378055No Hit
TCGAAGAGTCGAGTTGT509770.1457357547209242No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT506160.14470370875010885No Hit
AGAGGTGCACAATCGACCGATCCTGA503310.14388893561525462No Hit
GAGGTGCACAATCGACCGATC502620.1436916747510268No Hit
TTCCATCTAAAGCTAAATATT494260.1413016735554544No Hit
CCATCTAAAGCTAAATA489910.14005807245488744No Hit
ATACGAATAAGGCGTC484620.1385457391624738No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT474330.1356039793176844No Hit
TATAGATGGATACGAATAAGGCGTC472460.13506937378709583No Hit
CTTTGAACGCACATTGCGCCCCT471260.13472631141452565No Hit
ATCGACCGATCCTG467280.13358848787883448No Hit
TGTGGCGTCGCTGAACCATAG462630.13225912118512498No Hit
GATGGATACGAATAAGGCGT462180.13213047279541115No Hit
GTGGCGTCGCTGAACC457540.1308039649548064No Hit
GGGGGGCATGCCTGTTTGAGCGTCATT452510.12936596184311633No Hit
TGCAGCTCTAAGTGGGTGGTA437540.12508625874530313No Hit
AGCGGCAAAAGCTCAAATTT436150.12468887816374265No Hit
TGCAGCTCTAAGTGGGTGGT435140.12440013400016273No Hit
AATACTGCCAGCTGGGACT433770.12400847112481175No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGT433140.1238283633792124No Hit
TTCCATCTAAAGCTAAATATTG426170.12183574276520051No Hit
TGATGTTGACGCAATG423960.1212039362290504No Hit
ATAAATCATTTGTATACGACTTAGA409390.11703858725542726No Hit
TTTGAGCGTCATT408450.1167698550635806No Hit
CCTGTTTGAGCGTCATT395990.11320772409506007No Hit
GAGGTGCACAATCGACCGAT394050.11265310659273825No Hit
CCATCTAAAGCTAAATATTG391260.11185548657651255No Hit
TTTGGTGGCAGGATA386830.11058901465110756No Hit
ACTGCCAGCTGGGACTGAGGA382660.10939687290642613No Hit
ACGTGAAATTGTTGAAAGGG380160.10868215963023822No Hit
TGCCCGAGTTGTAATTTG371880.10631502925950388No Hit
TAGGGGGCATGCCTGTTTGAGCGTCAT371850.10630645270018962No Hit
TGGATACGAATAAGGCGTC364540.10421663108061616No Hit
TTTTAAAACCTAAAGGTAAACCTTTA362250.10356195371962805No Hit
TCAGTTTTGGTGGCAGGATA362200.1035476594541043No Hit
ATGATTAATAGGGACGGTCGGGGGC352930.10089750262599952No Hit
TTAGATGGATACGAATAAGGCGTC351970.10062305272794335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)