FastQCFastQC Report
Fri 30 Jun 2023
SRR1930188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32859094
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT7965562.42415691680361No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC5292571.6106865271452706No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT3295081.0027908864437953No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC3177850.9671143093598381No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2283850.6950435091119676No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC2152090.6549450206995969No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG2143860.6524403868225946No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1887300.5743615450870313TruSeq Adapter, Index 12 (100% over 49bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1457200.44346931780894505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG1454890.4427663160767609Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA1331760.40529419344306933Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC1063130.32354209157440555No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC1040730.31672510508049917No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT963930.2933525799585345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT922800.2808354971686073Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC909470.2767787815452246No Hit
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT785480.23904493532292764Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAA719700.21902612409216154Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT683540.2080215601805698Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT660420.20098545626364503Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT657890.2002155019855386Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC620660.1888853052369612No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA620580.18886095885662582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA589540.1794145632864984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC577320.17569565369026913No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG576140.17533654458032227No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAAGATCGGAAGAGCACACGTC570380.1735836051961749No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA553120.16833087363881669Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC543200.1653119224772296No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTC543040.16526322971655882No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA518420.15777063116834567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA512520.1559750856186114Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGT509720.1551229623068731No Hit
ATACCTAAGACAGACTATGATAACTTTCTTAGATCGGAAGAGCACACGTC475560.14472705790366588No Hit
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC468910.14270326503828742No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT455670.13867393909278203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATGGCCGAGAACCAGATTTGAGTTCAGACAGATCGGAAGAGCACACGTC445660.13562759825331763No Hit
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAACT441020.1342155081938656Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT438410.13342120753542383Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAAGAGATGAAGAGTGCTCGGAATTAGATCGGAAGAGCACACGTCT406270.12364004923568495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA403170.12269662699768898Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAAGAAAGACGTGGTCCTGACAGACAGAAGATCGGAAGAGCACACGTCT392090.11932465332123886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA387620.11796429931999952Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGAGATCGGAAGAGCACACGT362180.1102221503733487No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG357670.10884962318194165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCTGAA337120.10259564673329095Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)