FastQCFastQC Report
Sat 1 Jul 2023
SRR1930189.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930189.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37763856
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT13465333.5656660696937306No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8880012.351457435914383No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2712710.7183350132465287Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2615000.6924610664758387No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1970730.5218561367250208No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG1955420.5178019956436652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1885240.49921808832233655No Hit
GCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAA1866950.4943748329090123Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC1786060.4729548804550044No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1734470.4592936695871312No Hit
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA1732210.45869521375147704Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAAC1646200.43591946754589894Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1585660.4198882656474487No Hit
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT1321820.3500225188868425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC1235760.32723353250790915No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1180030.31247603528622714Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1130390.2993311911792058No Hit
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA1090530.2887761249804575Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1064660.28192565928648816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1019550.269980374885446TruSeq Adapter, Index 11 (100% over 49bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT896810.2374783973331537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAACT876150.23200755770279388Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC873280.23124757175220664No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT835910.2213518661865462Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA808580.21411478743060558Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT773810.20490757087941444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT763420.20215626285620833Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC736850.19512043473526644No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA730700.1934918934125795Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT724500.19185011191653734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA716210.18965489117424875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC692670.18342141755863067No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC683380.18096139334923847No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTC616080.16314012001316813No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG554020.14670641684472052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC535120.14170163131646302No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGT495910.131318687371332No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC490380.12985432419824924Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC485920.12867330073496733No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA476840.1262688852536669Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT454340.12031080724383654Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA453710.12014398105956128Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC449020.11890205279884553Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC427910.11331205160828915No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA425770.11274537218868752Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG383960.10167393922908718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)