FastQCFastQC Report
Sat 1 Jul 2023
SRR1930190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55650185
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAACT7286201.3092858541260914Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT7266461.3057386961067605No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC4861490.8735802046300475No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4418380.7939560308739315No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG4230400.7601771674254093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC3729680.6702008268256431No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2666230.47910532552587204No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG2299090.4131324990204435TruSeq Adapter, Index 10 (100% over 49bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT2080160.3737921086875093Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGCACGCGCGCGCGCGCGCGCGGACAGATCGGAAGAGCACACGTCTGAA2001220.3596070704886246Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA1731300.3111040870753619No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC1592090.28608889620043493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1532960.2754635945954178No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA1484240.26670890671792014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1428530.25669815832597864Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGT1419630.2550988824206065No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1407060.25284012982167087No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1325060.23810522822161326No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1161120.20864620665681524No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA1153120.2072086552811999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAA1084330.19484751039012718Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1033650.1857406224256038No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC1014090.1822258093122242Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA973760.1749787534399032Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG933950.16782513840699723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT928430.1668332279578226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA910140.16354662612532195Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC900750.16185930019819342No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC877010.1575933664910548No Hit
GGGAGAGCGACGCTCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGT872790.15683505814041768Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC861080.15473084231436068No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTC839140.1507883576667355No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA836570.15032654428731906No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC824090.1480839641413591No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCAGATCGGAAGAGCACACGTC819430.14724659046506314No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC792260.14236430660562943No Hit
GGGAGAGCGACGCTCAGACAGGCAGATCGGAAGAGCACACGTCTGAACTC779680.14010375706747427Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCGTCCCGGAGCCGGTCGCAGATCGGAAGAGCACACGTCTGAACTCCA752620.13524123953945527Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA751960.13512264155096698Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACGT734300.13194924688929605No Hit
ACGCCCGCGGTCGGCGGGAGAGGCCAGATCGGAAGAGCACACGTCTGAAC732650.13165275191807538Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAACT714800.1284452154112336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA694090.12472375428760928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAAC689880.12396724287619171Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC658860.1183931374172431Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC655760.11783608625919213No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC637290.11451713952074015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA622630.11188282662492498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC615980.1106878620439447No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAGATCGGAAGAGCACACGTCTGA598500.1075468122882251Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACACGTCT594470.10682264578275885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCTCGACTGCCGGCGACGGCCGGGTAGATCGGAAGAGCACACGTCTGA588280.10571034040587646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC571140.10263038658362052No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG563310.10122338317473697No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)