FastQCFastQC Report
Fri 30 Jun 2023
SRR1930191.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930191.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31240419
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT9465843.0299977730772434No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4230661.3542263949788893No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC3147881.0076305314599014No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2069000.6622830506850756No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1984750.6353147824297747TruSeq Adapter, Index 9 (100% over 49bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT1875090.6002128204490471Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG1619350.5183509222459531Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA1473180.47156217719102933Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT1470290.47063709356779115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1231010.3940440107413412No Hit
CTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAACT1169930.37449241637892244Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA1123640.35967507350013456Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAAC1116000.3572295237141346Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1045060.3345217616959619No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA1004530.32154818410085984Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC968630.31005666089177614No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC958860.3069293020685798No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG936240.2996886821524385No Hit
GCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAA925170.29614519574785475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC910340.2913981403386427No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA850210.2721506392087763Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA808040.25865210066484706No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC790660.2530887949998366Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA714660.22876133639564822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC689100.22057962794929223No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC671810.21504513111683937No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTC623450.19956518508922688No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA607220.19436999228467453Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT604590.1935281341777138Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC598270.19150511393589184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC550290.17614680520130027No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA511000.16357014929921393Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT483820.15486988186682132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC466320.1492681644250674No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTCTGAA444240.14220039750427163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT439310.1406223136763947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC436050.13957879374153082No Hit
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA410870.13151872258819577Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACGTC407250.13035996732310154No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT406950.13026393788124288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC406220.13003026623938685No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACACGTCT399280.12780878515105704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCAGATCGGAAGAGCACACGTC395740.1266756377371251No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACGTCTG391090.12518718138831622Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT380510.12180054307210156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA371010.11875961074657801Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTCTGAACT369310.11821544390937906Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACAAGAAAGACGTGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCT369220.11818663507682148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT358110.11463034474665658Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC351940.11265533922576391No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG349880.11199593705833459Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGCGGACGCTACGCCGCGACGAGTAGATCGGAAGAGCACACGTCTGAA347280.11116368189555972Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG339950.10881736253281366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATGGCCGAGAACCAGATTTGAGTTCAGACAGATCGGAAGAGCACACGTC319410.10224254674689223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)