FastQCFastQC Report
Fri 30 Jun 2023
SRR1930192.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930192.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36717313
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5575261.5184281050195585No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG3714201.011566396484405TruSeq Adapter, Index 8 (100% over 49bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG3474400.9462566065223782Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC3452900.9404010582146901No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2944220.8018615087656333No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2687270.7318808977116599No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA2302270.6270257303414333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAACT2007700.5467992715044263Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT1949710.5310056321387134Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1711300.4660744101835556No Hit
GCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAAC1506090.4101852442198044Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1241350.33808301822085945Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1179440.32122176260555885Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAA1138760.31014252050524505Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1120720.30522930694846867No Hit
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA1096080.2985185762367742Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC993640.2706189311837715No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA951540.25915295054406623Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTCTGAACT910170.24788578619573823Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC890760.24259945165377433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC808890.22030206840026664No Hit
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT785540.21394267058703342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG777550.21176658542524612Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC763040.20781477119526692No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG744710.20282257582410781No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG707340.19264481581209386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA688040.18738843988937862No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA668940.18218653418347905Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA663380.18067226215600254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTTGGCCCCGGATAGCCGGGTCCAGATCGGAAGAGCACACGTCTGAA656150.1787031638181149Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC645120.17569913136072893No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC625500.17035560309110856No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC610590.16629484842749795No Hit
GGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTC582350.1586036538131208Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC571050.15552608656303363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC543200.1479411088714471No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA538020.1465303302559204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCAGATCGGAAGAGCACACGTC527980.14379592537177216No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGAA527630.14370060249234468Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGA525570.14313955925914296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC509160.13867027796941458Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT486520.1325042494258771Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA473690.1290099850171498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACGT438250.11935786259740738No Hit
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC424200.1155313298661043Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC408730.11131805859540973No Hit
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT394600.10746973777738039Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT390290.10629590460500199Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTAGATCGGAAGAGCACACGTCTGAAC388660.10585197233795403Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGCGGACGCTACGCCGCGACGAGTAGATCGGAAGAGCACACGTCTGAA374870.10209625088851138Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC367950.10021158138668808Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC367570.10010808797473825No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC367260.1000236591386739No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)