FastQCFastQC Report
Sat 1 Jul 2023
SRR1930193.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930193.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65933295
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT8692441.3183688150273698No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG7001811.061953600225804TruSeq Adapter, Index 7 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6504450.9865197848825847No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG5890830.893452996699164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC5175570.784970628269071No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC4769260.7233462243923954No Hit
GCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAAC3154910.47850027819783614Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA2503400.37968677282092456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2499700.3791255995927399No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2424650.36774288316699477No Hit
GCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAA2416680.36653408569979706Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAACT2394910.3632322637599107Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT2058550.31221706726472565Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1795450.27231310068759645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA1768500.26822563622825163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1727750.2620451472962181No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC1685410.25562350554450525No Hit
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT1576880.2391629297458894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA1423050.21583177361301298Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1351910.20504208078786293Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTCTGAACT1321740.2004662439515574Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC1279660.19408403599425753No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1220110.18505218038928586No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1178660.17876552354921743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1065220.16156025570995655Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1044230.1583767351533091Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC1029610.15615934255977956No Hit
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT1002840.15209917841964368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA981240.1488231401145658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGA934030.14166287305980993Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC912570.138408068336339No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT900870.13663354758775517Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA895420.13580695458948322Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA884650.13417348549014577Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC876020.13286458685251512Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC864530.13112191647634172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC838280.1271406199250318No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCAGATCGGAAGAGCACACGTCTGAA827130.1254495168184754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT816750.12387519841075742Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGAA777060.1178554780251768Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA757980.11496164418902469Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG699700.1061224075029164No Hit
CTCGGTTGGCCCCGGATAGCCGGGTAGATCGGAAGAGCACACGTCTGAAC668100.1013296847973395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)