FastQCFastQC Report
Fri 30 Jun 2023
SRR1930194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44272766
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT14686523.3172808764647774No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8023211.8122224394111721No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC7951031.7959189629127756No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG4611731.0416629491818965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA4440851.0030658576877713No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG3719560.8401462876749106TruSeq Adapter, Index 6 (100% over 49bp)
GCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAAC3564700.8051676735083595Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG3267000.7379254325333998No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT3112430.7030123213896327Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAACT3034970.6855162381315864Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2808550.6343741884119009Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC2789620.6300984221315651No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA2226350.5028712233611065Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA2218930.5011952494678105Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2096760.4736004070764406No Hit
GCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAA2019580.4561675681162546Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1934940.43704972036307826No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1698000.3835314920237873No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1641980.37087811500189527Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1590760.3593089259433214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC1578160.35646293253961137Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1272110.28733465625346294Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1154540.2607788273269396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTCTGAACT1091250.24648335728560533Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA1062530.2399962993050852Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1043900.23578829477245672Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC1043370.23566858235150703Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT1007440.22755298370108612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACTC986980.2229316325074426Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG958680.2165394409737128No Hit
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA955640.21585278859694465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGAA883870.19964191982041513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTCTGAA806530.18217294126145178Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT783930.17706822293416227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAACT782100.17665487627314724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGA767960.17346103923120593Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG742890.16779841584779231Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG701860.15853086748634587Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGCGGACGCTACGCCGCGACGAGTAGATCGGAAGAGCACACGTCTGAA696730.15737214160054966Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC692570.1564325120323406No Hit
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC691490.15618856974059403Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA653490.1476054150309922No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG648610.14650315726828544Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACGTCTG644230.14551383575175764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACACGTCT639710.14449289208629976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTC631380.14261137422495807Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGTTGGCCCCGGATAGCCGGGTCCAGATCGGAAGAGCACACGTCTGAA588220.13286271745478923Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT567330.12814424108943182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA518810.11718490776022443Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC512370.11573028890943927No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCAGATCGGAAGAGCACACGTC508730.11490811303725636No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG502930.1135980525815803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTAGATCGGAAGAGCACACGTCTGAAC484970.10954138261883163Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACGTC479920.10840072653242401No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAAC473440.10693707278194453Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT469200.10597937341434688Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA464630.10494713612427106Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA462110.10437793744352906No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)