FastQCFastQC Report
Fri 30 Jun 2023
SRR1930195.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930195.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40399279
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT15941213.945914480305453No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC11106962.7492965901693442No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC5036621.2467103682716714No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA4087311.0117284518864804Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAAC3588610.8882856548009186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2980390.7377334630155158TruSeq Adapter, Index 5 (100% over 49bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2481610.6142708635963527No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2365370.5854980728740233No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2260690.559586719357046No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2087070.5166107048593614No Hit
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA1996810.49426872197397387Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT1986390.4916894680224367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG1928010.4772387150770686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAA1852480.45854283686597475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1567050.38789058586911906No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1542580.3818335470788971No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1521190.37653889813231567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA1372150.3396471506335546Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1314090.3252756070225907No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC1202000.2975300623558158No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1115270.27606185744057465Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1051620.26030662576923713No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT983380.24341523520753922Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTC929730.2301352952363333No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT885290.21913509892094854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT880260.2178900271957824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAACT878990.21757566514986568Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA877750.21726872897904936Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA845240.20922155566192158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA838720.2076076654734358Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT777150.19236729447572568Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT746050.18466913728831647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGCGGACGCTACGCCGCGACGAGTAGATCGGAAGAGCACACGTCTGAA730590.18084233631991303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA729850.1806591647341033Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC712510.17636700892607515No Hit
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC712410.17634225600907383No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC700290.17334220246851437No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT640020.1584236193918213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC637530.15780727175848858Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC632540.15657210120012288No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC587290.14537140625702752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA576310.14265353597028302Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG571930.1415693582056254Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC538890.13339099442839067No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC529510.13106917081366726No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT512720.12691315604914633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA500960.12400221300979158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTCTGAACT496250.12283635061902962Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATGTCTTGGAGAATTCCTTAGTTCTTAAAGATCGGAAGAGCACACGTCT452340.11196734476375185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT446990.11064306370418145Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG444140.10993760556964394Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTGTGTCCATGCAGAAGAAGCTAGTTCTAGATCGGAAGAGCACACGTC439690.1088361007630854No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA430010.10644001839735803No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG423720.10488305991797527No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCT423030.10471226479066618Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA409770.10143002799629171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)