FastQCFastQC Report
Fri 30 Jun 2023
SRR1930196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36720238
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT11742643.1978659833305No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8132042.214593489290565No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2699010.7350197457870508TruSeq Adapter, Index 4 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2589930.7053140559709881Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2124860.5786618267561338No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2043710.5565622967912136No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1904300.5185968565890015No Hit
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT1847970.5032565420736107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG1769170.4817969861742181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1713750.4667044914033509No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1504920.4098339449760647No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1476200.402012644907149No Hit
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA1338630.3645482907817754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1202550.32748970744688527No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1169900.31859815287689586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAA1119020.30474203353475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC1113310.30318703272021275No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1024410.27897694998599953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA956140.26038502255894963Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAAC954710.2599955915318414Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT915430.24929849310889543Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTC875880.23852786575076118No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA864530.2354369271789578Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA832030.2265862220174063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT761570.20739789322716265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT749600.2041381104338158Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA747840.20365881070814412Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT679620.1850804997505735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC678080.18466111249061076No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC657930.17917367529044884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC643670.17529025819494962No Hit
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC632770.17232186784846001No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT614010.1672129684998229Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC594790.16197879763197612No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC564660.15377351312374393Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC552430.1504429246891047No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT550500.14991732896720333Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT486010.13235480663278926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA449800.12249375943587293No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA445590.12134725270571503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC445590.12134725270571503No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG431290.11745294243463236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAACT420810.11459893043176898Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACAAGAAAGACGTGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCT420630.11454991114164348Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGTCTTGGAGAATTCCTTAGTTCTTAAAGATCGGAAGAGCACACGTCT408470.11123838576427528Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGTGTCCATGCAGAAGAAGCTAGTTCTAGATCGGAAGAGCACACGTC406130.11060113499264358No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG398670.10856955774633051Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA397690.10830267494453603Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG395480.10770082699355053No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT392690.10694102799660504Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG392350.10684843600414573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGCGGACGCTACGCCGCGACGAGTAGATCGGAAGAGCACACGTCTGAA379060.10322917841654512Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC375610.10228964202247273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)