FastQCFastQC Report
Fri 30 Jun 2023
SRR1930197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39308948
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC8182462.0815769478236863No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA3815260.9705830845434988Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3715030.9450850732510063Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT3552020.9036161435813546Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC3191140.811810074388152No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG3060090.7784716090595962TruSeq Adapter, Index 3 (100% over 49bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT2227820.5667462787353149Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1776880.45202939544451815Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA1681060.4276532661214948Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT1663330.4231428426932209Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1594330.4055895873886017No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC1474150.3750163957580345No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1381730.3515052094500214Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1314970.33452179895529127No Hit
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT1201610.3056835812548329Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGTGTTGCCGATGAGTCTGCCGCCAAAGATCGGAAGAGCACACGTCT1178090.29970021075099745Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1169600.29754039716351605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1062990.27041934574285736No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGAAGATCGGAAGAGCACACGTCT1054380.2682290047548462Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1041690.2650007321488227No Hit
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAACT971960.24726176849098072Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG873280.22215806945533115No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAAGATCGGAAGAGCACACGTC809720.2059887229747283No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG790880.20119592109155401Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCTAAGACAGACTATGATAACTTTCTTAGATCGGAAGAGCACACGTC688350.17511280128890755No Hit
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA646220.16439513975291326Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA619740.15765876003601012Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC583590.14846238062641615No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC538970.13711127552942906Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC536970.13660248552059953Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCACAAGAAAGACGTGGTCCTGACAGATCGGAAGAGCACACGTCTGAAC520840.1324990940993893Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAGATCGGAAGAGCACACGTCT508120.1292631896432334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGAAGATCGGAAGAGCACACGTCTG505030.12847710907959176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAAGAGATGAAGAGTGCTCGGAATTAGATCGGAAGAGCACACGTCT500670.12736794686034333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT431620.10980197180550343Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGATTAAAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCTG427260.10869280958625502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG420660.10701380255711754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAAGAAGACACCCTGATTACTCTGTATCAGATCGGAAGAGCACACGTCT416740.10601657413981164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA412100.1048361813193271Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAGACGTGGTCCTGACAGACAGACAATCAGATCGGAAGAGCACACGTCT398810.1014552717106548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)