FastQCFastQC Report
Sat 1 Jul 2023
SRR1930198.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930198.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44632590
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC14919003.342624750210552No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA5684491.273618671916642Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC5272441.1812982396943579No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT5133731.150220052208487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG3585620.8033636407835619TruSeq Adapter, Index 2 (100% over 49bp)
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT3302690.7399727418910711Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2569520.5757048829117916No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2446390.5481174182363157Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT2210010.4951561179846386Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2060740.46171194636027174No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2037240.45644673544600484No Hit
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT2030240.454878374748138Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC1917970.4297241096696383No Hit
ACGTGTGTTGCCGATGAGTCTGCCGCCAAAGATCGGAAGAGCACACGTCT1825440.4089926217591226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAAGAAAGACGTGGTCCTGACAGACAGAAGATCGGAAGAGCACACGTCT1644110.3683653581385261Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA1398510.3133383027962303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1320490.29585780256086414No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAAGATCGGAAGAGCACACGTCTG1209400.27096791828571903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1207040.27043915667900964No Hit
ACTGTAAGAGATGAAGAGTGCTCGGAATTAGATCGGAAGAGCACACGTCT1151150.2579169167641851Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCTAAGACAGACTATGATAACTTTCTTAGATCGGAAGAGCACACGTC1096970.24577780496269655No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAAGATCGGAAGAGCACACGTC946170.2119908345000817No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC925150.2072812713759161No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA911340.20418711977055332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCACAAGAAAGACGTGGTCCTGACAGATCGGAAGAGCACACGTCTGAAC874850.19601147950410228Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAGATCGGAAGAGCACACGTCT833860.1868276073604512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGAAAAGGCTGGTGAATATTCTGTGACAGATCGGAAGAGCACACGTCT823890.18459381362363242Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC757310.16967646287163707Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG737080.16514390045480218Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT726390.16274878961763142Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA679310.15220044366683627Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAAGAAAGACGTGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCTG653240.146359420324924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAA619440.13878647866951033Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAAAGTCCTACGTGATCTGAGATCGGAAGAGCACACGTCTGAACT580420.1300439880365446Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAAGAAGACACCCTGATTACTCTGTATCAGATCGGAAGAGCACACGTCT548280.1228429719180536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTCTGA528720.11846052402515742Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAACT505660.11329389578332784Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATACCTAAGACAGACTATGATAACTTTCTAGATCGGAAGAGCACACGTCT488430.10943348795129298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGACGTGGTCCTGACAGACAGACAATCAGATCGGAAGAGCACACGTCT476020.10665300848550353Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)