FastQCFastQC Report
Sat 1 Jul 2023
SRR1930199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69378421
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT18274982.634101459299571No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC7697081.1094343009045997No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6119330.8820220915664829No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA6110170.8807017963121414No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA6098400.8790053033925348No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT4600210.66306063667837No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG3238230.4667488757058913No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2748840.39620965141308134No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG2383150.34350017853534026No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2280750.32874054599772456No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC2125820.3064093949327558No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1774610.25578702634353695No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1561400.22505556879133928Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1535450.22131521269416035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1532490.22088856706611987Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC1303590.18789559941123482No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1291120.1860982105660779No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCAGATCGGAAGAGCACACGTC1184590.17074329206771657No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGGAAGAGCACACGT1174570.169299038961985No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1157760.16687609537841744No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC1029890.14844529252114286No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1010370.14563173756865985No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1002910.14455647527636872No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC936090.13492523849742846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC935740.13487479053465343Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT920020.132608956320871No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT857180.12355138494720137Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC788970.11371979768752592No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG763120.10999385529399695Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC726970.10478330142451643No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT711440.10254485324766903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)