FastQCFastQC Report
Sun 2 Jul 2023
SRR1930200.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930200.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33419619
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5966051.7851939006246602No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3368671.0079917428143033No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2150200.6433945282260698No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1671170.5000565685683012No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1532200.45847321000278307No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC1287880.385366451963441No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1223720.36616814811682924No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1198390.35858876787314664No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1164140.3483402967580211Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1111030.3324484339573111No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG904970.2707900410235078TruSeq Adapter, Index 12 (100% over 49bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT796160.23823132154798055Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT778920.23307267506550566Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG771930.230981089281718No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA760350.2275160587557865Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC723300.21642975642540987No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT718890.21511017226138934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT665480.19912854183047388Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC628320.18800932470235523No Hit
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC616540.18448444908962008No Hit
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT612010.18312895787351735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT566610.1695441231690882TruSeq Adapter, Index 12 (100% over 35bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT520610.15577975320424806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC505280.1511926273007481No Hit
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC489600.14650077249534174No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTC482820.1444720240526979No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC478180.14308361803885317No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA414320.12397508182244686Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT411980.12327489430684413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCTAAGACAGACTATGATAACTTTCTTAGATCGGAAGAGCACACGTC410340.12278416459505419No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA406650.1216800227435268Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG365270.10929807428385105Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC361810.10826275428214786No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA358110.10715562017628029Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA344870.10319387542987847No Hit
CATACTGTAAGAGATGAAGAGTGCTCCGAAGATCGGAAGAGCACACGTCT343100.10266424641166615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGAAGATCGGAAGAGCACACGTCTG339550.10160199612090132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)