FastQCFastQC Report
Fri 30 Jun 2023
SRR1930201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35633658
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT8339232.340267732265938No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC5513931.5473937590128974No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1866220.5237239466124977No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC1844080.5175107197807197No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1495730.4197520220910242No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1319950.3704222563959052No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1205890.3384131935037374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1126890.31624314292964256No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1055020.2960740095782476Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT979050.27475427866541235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC971270.2725709496341914No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA886280.24871990408618727No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT874970.2455459386179213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC827630.2322607462865586No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT761500.2137024495211802Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT731630.2053199253357598Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT677290.1900702981434014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG628370.17634170480055683No Hit
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT604130.16953914751048013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA576210.1617038587506228Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA543800.1526085253442125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC542600.1522717650823275No Hit
CATACTGTAAGAGATGAAGAGTGCTCCGAAGATCGGAAGAGCACACGTCT513050.14397904363340974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA501410.14071246909312538Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC496020.13919985425015866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC478480.13427754175560647No Hit
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT425420.11938712550925867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC417140.11706347970225228No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG413690.11609529394933295TruSeq Adapter, Index 11 (100% over 49bp)
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG411240.11540774174798445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC396150.11117298145478076No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC376570.10567817651502408No Hit
TGTAAATGAAGAGTGCACCGAAGATCGGAAGAGCACACGTCTGAACTCCA368790.10349484748380311Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA368040.10328437232012498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACAAGAAAGACGTGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCT358520.10061274090917077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)