FastQCFastQC Report
Sat 1 Jul 2023
SRR1930202.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930202.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44333775
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5630201.269957272982055No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3065350.6914254425660797No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG2547450.5746070574860814TruSeq Adapter, Index 10 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2061060.46489612039579303No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1888060.42587395275949325No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC1638820.36965496396370484No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1624130.36634146313955895No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1500930.33855226630261015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1470950.33178992765673576No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1258180.2837971726973397Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1253090.28264906383451444No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1252320.28247538135428346Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC1086630.24510206947186428No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT965440.21776625157681698Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG959560.2164399490005081No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA895650.2020243031413409Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT856190.19312363993366233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT811420.18302524429737824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC795660.17947039249421012No Hit
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT762860.17207196995969776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG717390.16181568115956738No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC705250.15907736257514726No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC703890.15877059871396018No Hit
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC702840.15853375896819072No Hit
ATACCTAAGACAGACTATGATAACTTTCTTAGATCGGAAGAGCACACGTC615580.13885124828643625No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC601140.13559413787794972Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC568900.1283220298745144No Hit
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT566940.12787992901574477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT540460.12190705618910187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA508110.11461013640277644Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTGAAC501700.1131642861452696Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG480690.10842523561325422Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG479370.10812749421857262Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAAGAGATGAAGAGTGCTCGGAATTAGATCGGAAGAGCACACGTCT475170.10718013523549484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)