FastQCFastQC Report
Sun 2 Jul 2023
SRR1930204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45089631
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT8912021.9765120721435931No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4401770.9762266628440583No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC3385360.7508067653070836No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2944550.6530437119789247No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2402560.5328409096982851No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1551740.3441456418217306Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1509630.33480646581472356No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1409130.3125175275885491No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1146220.25420922162791704No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1098840.24370126249203505No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC993290.2202923328425553No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG991220.21983324724924005TruSeq Adapter, Index 8 (100% over 49bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC987160.2189328185009986No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA836290.18547279750415346Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT816260.18103053449250892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC770560.17089516656279577No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC758860.16830033494840532No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT697600.15471406275203273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA668960.14836226980877268Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT624640.13853295894126966Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC618850.13724884996286618Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC615270.13645487584495866No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT612280.13579175221017Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA594080.13175534747667375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC546080.12110988444327699Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA543990.12064636324036451Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG542710.1203624842261406Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC534510.11854388429126865No Hit
CGCGACCTCAGATCAGACGTGGCGAAGATCGGAAGAGCACACGTCTGAAC512630.11369132739187864Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG497610.11036018458434489No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC487960.10822000295367244Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT480670.1066032232554753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC473750.10506850233482727Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT460370.10210107951426793Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA458690.10172848830809902No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG457900.10155328172900771Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)