FastQCFastQC Report
Sun 2 Jul 2023
SRR1930205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52993962
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT8391541.5834898322944793No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4561950.860843354191936No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2911250.5493550378437453No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2325130.438753758399872No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2258410.42616364483183955Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2085590.3935523824393428No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1480540.2793789979318776No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1327170.25043796498929444TruSeq Adapter, Index 7 (100% over 49bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1281090.2417426347552576No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1274830.2405613681045399No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC1270590.23976127695453306No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1122140.21174865166714651No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1102860.20811050134353043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1049720.19808294386443495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC923030.1741764467431214No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT749900.1415066871203176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT722410.13631930369727782Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT693920.13094321953131188Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC687370.1297072296651456Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC679720.12826366898176061No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC612110.1155056117525238No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC590350.11139948358645085Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT588940.11113341553892499Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC572570.10804438437722395No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC556560.10502328548297635Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT540130.10192293227669975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC537350.10139834421136505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)