FastQCFastQC Report
Sat 1 Jul 2023
SRR1930206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47166047
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT10410522.2072063830153077No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8132231.7241703550013425No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2776430.5886501364000253TruSeq Adapter, Index 6 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2098850.444991711940583No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2055700.4358431818549475No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1760240.37320066275641034No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT1341320.28438253475005015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1257610.2666345984008369Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC1225550.2598373359548236No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1170430.24815096334021802No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC1153110.244478830290781Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1108500.23502075550236382No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1104150.23409848190161028No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1044480.2214474323023085No Hit
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT993560.2106515307504994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT984020.20862888933643303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA940390.19937859112933504Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA838120.1776956207502401Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC717510.1521242600636004No Hit
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT658190.1395474163861983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT654470.1387587134448643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAACT594530.12605041927723984Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT589240.12492884977195566Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG587530.12456630083924566No Hit
GTGCAGGAAGTAACAGACTTTGCAAAGACGAGATCGGAAGAGCACACGTC565160.11982348234525568No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC557690.1182397159549962No Hit
ACGTGTGTTGCCGATGAGTCTGCCGCCAAAGATCGGAAGAGCACACGTCT540320.1145569820595735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCCACCATTTGAAAGGCCAGAGGCTGAAGATCGGAAGAGCACACGTCT539270.11433436429387436Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC533570.11312586785150767No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA530080.11238592880170772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTAAATGAAGAGTGCACCGAAGATCGGAAGAGCACACGTCTGAACTCCA528670.11208698494491175Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC496790.10532788554444683No Hit
CTGACTTTAGTCTGTATCCATGCAGAAGAAGATCGGAAGAGCACACGTCT491900.10429112280704804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC491040.10410878825609447Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT480710.1019186534754545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)