FastQCFastQC Report
Sat 1 Jul 2023
SRR1930207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48413026
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG31539356.514641328141728TruSeq Adapter, Index 5 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT17759233.668275145618867No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC10569422.1831768995393923No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC4859501.003758781779102No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT3850110.7952632417564646No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2938450.6069544175982721No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2635420.5443617591678735No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2365440.4885957758558616No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG2148010.4436843092600739TruSeq Adapter, Index 5 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT1611330.33282984624840434Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1570890.3244767224424269No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1423840.29410266567514287No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1423290.29398905988648594No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1350290.2789104733920164No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT1138500.23516398251991108TruSeq Adapter, Index 5 (100% over 50bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1057470.21842675151104995Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC986620.20379226037223946No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT982160.20287102070422122Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT829260.1712886114575858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT824130.17022897928338543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT751850.15529911309406688Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA727760.15032317955089194Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG723200.14938128428493602No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA707090.14605366745718396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC704640.14554760530771202No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC684840.1414577969160614TruSeq Adapter, Index 5 (100% over 50bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC670000.13839250618211718No Hit
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC650610.13438738574201084Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC616830.12740992475867963TruSeq Adapter, Index 5 (100% over 50bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT602040.12435496182370423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA588730.12160570173820574No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA574260.11861683671663076Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT553440.11431634122601633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC529680.10940857115603556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT499520.10317884281804653Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)