FastQCFastQC Report
Fri 30 Jun 2023
SRR1930208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42240734
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4952931.172548280055929No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2616050.6193192570943488No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2596320.6146484102288564No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1737980.41144644882354553No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1424260.33717690606417966No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1383450.32751561561406584No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1230170.29122836738585084TruSeq Adapter, Index 4 (100% over 49bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT1075010.25449605113395996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1050990.24880959691656873No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC1036470.24537215664860368No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1020440.24157724153183513No Hit
CCTCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT987950.23388561382479764No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC781130.18492339645423775No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC744970.1763629391477904No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT690390.1634417621625609Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG674810.159753379285502No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC660210.1562969999526997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT649310.15371655236862125Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC563530.1334091400968553No Hit
CCTCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC545510.1291431157422596No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC537550.12725867879095093No Hit
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC522350.12366025647186907No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTC495760.11736538479658047No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT482880.11431619535777952Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC458610.10857055656277184No Hit
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC438530.10381685128861634No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC435960.10320843383071895No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)