FastQCFastQC Report
Fri 30 Jun 2023
SRR1930213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30851029
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT13910214.508831779970775No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8616392.7929019806762363No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2569700.8329381817377955No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2301650.746052911233528No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1909390.6189064228619409No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1745560.5658028456684541TruSeq Adapter, Index 2 (100% over 49bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1437420.4659228708384411No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1330230.43117848678564336No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT1294370.4195548874561039Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1102220.3572717136922726No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTC1028930.33351561790694245No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG905850.29362067631520494No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA826150.2677868540462621Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATCTCATTAACGAAAAGGATGGGGAAACAGATCGGAAGAGCACACGTCT722790.23428391967088036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA702180.22760342937021646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA647640.2099249266531758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC628860.203837609435977No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA571640.18529041608304214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTAAGAGATGAAGAGTGCTCCGAATTAGATCGGAAGAGCACACGTCT562420.18230186098492857Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT538680.17460681781473156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAAAGATCGGAAGAGCACACGTCT514280.16669784336853075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC492230.15955059392022225No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC482070.15625734882295175Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCT471860.15294789681083248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT425700.13798567302244602Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG415440.13466001409547798No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT402030.1303133195330373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGAAGATAATGGCAACTTTAGACTTTTAGATCGGAAGAGCACACGTCT354150.11479357787385308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTC343260.11126371181979051No Hit
TCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTCTGA340290.11030102107777344Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTAAGACAGACTATGATAACTTTCTTATGAGATCGGAAGAGCACACGTC338350.10967219278164109No Hit
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC329350.1067549481088621No Hit
AACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG325730.105581567473811Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG319310.10350059960722865Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGAGGAGCATGGAATCCTTAGAGAAAATAGATCGGAAGAGCACACGTC315330.1022105291852664No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA313010.10145852833628338Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)