FastQCFastQC Report
Fri 30 Jun 2023
SRR1930214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10824709
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5781395.340919557283249No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3598103.3239692632845834No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1280801.1832188745212457No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC1112141.0274086813788712No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC636050.587590853481604No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA567930.5246607553145308No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG558780.5162078721931462No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT558640.5160785384623273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC545850.5042629783396486No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG522110.48233167284219836TruSeq Adapter, Index 1 (100% over 49bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG441760.4081033494757226No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA324250.2995461587004325Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC292410.27013197306274006No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA292210.2699472105901415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG223640.20660139685972156No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT208340.19246706770593092Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA201360.18601885741224083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT190100.1756167302049413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA188690.17431415477312137Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT175550.1621752603233953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG169970.1570203873378952Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGAAGATCGGAAGAGCACACGTCTGAAC165280.1526877073554587Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC160530.14829959863124265Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG158070.14602702021828023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCAGGAAGTAACAGACTTTGCAAAGACGAGATCGGAAGAGCACACGTC154520.1427474863296556No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA146950.1357542267417997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC131470.12145361136267036No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG127090.11740731321276164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCACCAGAAAGACGTGGTTCTGACAGGCAAGATCGGAAGAGCACACGTC123550.11413701744776696No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTAGATCGGAAGAGCACA120700.11150415221323734No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTAGATCGGAAGAGCACACGTCTG120460.11128243724611905Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA119090.11001681430881884Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA118950.10988748057799984No Hit
ACGTGTGTTGCCGATGAGTCTGCCGCCAAAGATCGGAAGAGCACACGTCT115940.10710680536539134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC115150.10637699359862698Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA111960.1034300321606798Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG108590.10031678449739388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)