FastQCFastQC Report
Sat 1 Jul 2023
SRR1930236.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930236.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40633578
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG506621912.468060282557445TruSeq Adapter, Index 7 (100% over 49bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1785530.43942229256798404TruSeq Adapter, Index 7 (97% over 49bp)
AACCAAAAACCTTGGTGCAAATCCAAATAAAAGTACCAAGATCGGAAGAG1025310.2523307201743346No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC998500.2457327287299189TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC847690.20861810397302447TruSeq Adapter, Index 7 (100% over 50bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG823240.20260091296907204TruSeq Adapter, Index 7 (97% over 49bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG743580.18299643708462002TruSeq Adapter, Index 7 (100% over 50bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG660070.16244446895619186TruSeq Adapter, Index 7 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA485450.11947015839953842No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT445580.10965807638205034TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)