FastQCFastQC Report
Fri 30 Jun 2023
SRR1930237.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930237.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40077965
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG9022652.251274484620165TruSeq Adapter, Index 8 (100% over 49bp)
AACCAAAAACCTTGGTGCAAATCCAAATAAAAGTACCAAGATCGGAAGAG1668600.4163385042129759No Hit
TGGAGAAGGTTCTGGGTTCGATTCCCAGCTACTCCACCAAGATCGGAAGA1195150.29820625872596074No Hit
AAGCCCGTCGTTGCCTAGATTTGTAGATCGGAAGAGCACACGTCTGAACT994620.24817128314773468Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA985220.24582585468099488No Hit
AGCCCGTCGTTGCCTAGATTTGTAGATCGGAAGAGCACACGTCTGAACTC765590.19102516806928693Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGTAAGCCCGTCGTTGCCTAGATTTGTAGATCGGAAGAGCACACGTCT755640.18854250708587625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGCCCGTCGTTGCCTAGATTTGTAGATCGGAAGAGCACACGTCTGAAC716820.17885638654557434Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCAAAAACCTTGGTGCAAATCCAAATAAAAGTACCAAGATCGGAAGAGC546440.13634424801758274No Hit
GTAAGCCCGTCGTTGCCTAGATTTGTAGATCGGAAGAGCACACGTCTGAA483410.12061740160709258Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACAGATCGGAAGAGCACACGTCTGAAC467320.1166027267103008Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAACTGACTTCCAATTAGTAGATTCTGAATAAACCCAGAAGAGAGTACC445720.1112132315101328No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)