FastQCFastQC Report
Sat 1 Jul 2023
SRR1930238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50481958
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG39057977.73701566805313TruSeq Adapter, Index 9 (100% over 49bp)
AACCAAAAACCTTGGTGCAAATCCAAATAAAAGTACCAAGATCGGAAGAG3251410.6440736708350338No Hit
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1722060.3411238526049247TruSeq Adapter, Index 9 (97% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA933470.18491160743012383No Hit
ACCAAAAACCTTGGTGCAAATCCAAATAAAAGTACCAAGATCGGAAGAGC853620.16909407515453342No Hit
ATAACTGACTTCCAATTAGTAGATTCTGAATAAACCCAGAAGAGAGTACC529810.1049503666240521No Hit
AGATCGGAAGATCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG520370.10308039161238557TruSeq Adapter, Index 9 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)