FastQCFastQC Report
Sat 1 Jul 2023
SRR1930239.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930239.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30974861
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG30475789.838875467431476TruSeq Adapter, Index 9 (100% over 49bp)
AACCAAAAACCTTGGTGCAAATCCAAATAAAAGTACCAAGATCGGAAGAG1759840.5681510564325051No Hit
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1111580.35886521008116873TruSeq Adapter, Index 9 (97% over 49bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG510160.16470130406719177TruSeq Adapter, Index 9 (97% over 49bp)
ACCAAAAACCTTGGTGCAAATCCAAATAAAAGTACCAAGATCGGAAGAGC494300.15958102281718067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA442420.14283195653404224No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG374270.12083024359657336TruSeq Adapter, Index 9 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)