FastQCFastQC Report
Sat 30 Sept 2023
SRR19424369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19424369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14749876
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC4365112.959421489373877No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT3574962.4237220706126616No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCCGTAAGCATCTC3114892.1118075840095196No Hit
TGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG2932501.988152307178718No Hit
TCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA2802301.8998803786553866No Hit
TCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2740361.8578868052856852No Hit
TGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA2662771.8052829732263513No Hit
TCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2113631.4329815382854743No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG1794941.216918704943689No Hit
TTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1766321.1975151519917862No Hit
TCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1735941.1769183686696756No Hit
TCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1725741.17000305629688No Hit
TCCCCGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCA1266190.8584411150303908No Hit
TGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGC1253060.8495393452799196No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1238510.8396748555716671No Hit
TCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1227570.8322578440659433No Hit
TCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA1158970.785748978499887No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1079640.7319654755063704No Hit
TGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTT1056000.7159382221247148No Hit
TGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACAC1018960.6908261466062495No Hit
TGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1018520.6905278390136975No Hit
TGCCGCGCGGAACCGCGGCCCCGGGGGCGGACCCGGCGGGGGGAGACCGC1016030.6888396892285739No Hit
TCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG784720.5320180318804036No Hit
TGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC750080.508533088684949No Hit
TGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCC729090.49430246057661775No Hit
TCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG705850.47854639591546394No Hit
TCCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTA673650.4567157039150702No Hit
TGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA669210.4537055091175004No Hit
TTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA655460.44438339685025147No Hit
TGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC651410.4416376110551709No Hit
TGGGAGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGCCACGGCGCAC651260.4415359152849827No Hit
TGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGCA631080.42785444433566766No Hit
TGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACA620310.42055268803615703No Hit
TCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGGGCTGTAACACCCGGG617300.4185119929143811No Hit
TGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA584910.396552486271749No Hit
TGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCG581270.39408466891518273No Hit
TGGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGG579920.39316940698348923No Hit
TGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG571560.38750156272500186No Hit
TAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCC566340.3839625499224536No Hit
TTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT559870.3795760723683372No Hit
TGTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA542680.367921737104773No Hit
TCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGG541310.36699291573705434No Hit
TCACAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCG523210.35472162613434854No Hit
TGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGC509280.3452774789428738No Hit
TCCCGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCAC496210.336416387500478No Hit
TGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC489440.33182651840598526No Hit
TGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT472920.32062642424926147No Hit
TGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGGA454870.30838903323661837No Hit
TCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC454630.3082263200043173No Hit
TAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCG452050.3064771527570808No Hit
TGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGCAA445810.30224660871725295No Hit
TGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAG432860.2934668738910076No Hit
TCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGAT417010.2827210208411244No Hit
TTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC415490.2816905037032176No Hit
TGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTAT408210.27675486899008506No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC405400.27484976822856005No Hit
TCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG395900.26840903611664263No Hit
TTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT394130.26720902602842217No Hit
TGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTG391470.2654056210370853No Hit
TGGCGGCGACACCGGCGCGCCCCCGCGGGGGGAGCCCCCTCGCGGGGGAC387690.26284288762834346No Hit
TCTGCTGCTGCCGGGGCGGGGCTGTAACACCCGGGGAGGGGTCGCGCCCG379380.25720894195991884No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCTCTTATACACA366180.2482597141833599No Hit
TCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGG364770.24730377394359113No Hit
TACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT362670.24588003316095677No Hit
TCCGCGCGGAACCGCGGCCCCGGGGGCGGACCCGGCGGGGGGAGACCGCG361720.24523595994976502No Hit
TCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT361210.24489019433112524No Hit
TTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG352280.23883590614592284No Hit
TGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACACCGGCGCGCC348870.2365240223036451No Hit
TAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTT337620.22889683953953238No Hit
TGTCCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGGACCGAC334450.22674766892955572No Hit
TGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGCAAGGCCGG330960.22438154734317767No Hit
TGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGA330960.22438154734317767No Hit
TGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGC322940.21894421349711685No Hit
TGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAG319190.21640181924241264No Hit
TGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACACCGG311250.21101872314045217No Hit
TGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC310630.21059838062367442No Hit
TCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGGACCGACGGG308490.20914752096898984No Hit
TCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGC301260.20424578484592007No Hit
TAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG297510.20170339059121584No Hit
TCTTTACTAACACCAACTCCTAACCCTACTAAAACCCTCTAATCCTCTAT294570.1997101534955277No Hit
TCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGGGCTGTAACACCCGGGG292700.19844234622718185No Hit
TGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGC290960.19726267529299907No Hit
TGCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT276620.18754055966301006No Hit
TCGGGCCCATACCCGGCCGTCGCTGGCAGTCGGCAGAGCGAGAAAGAGAG270640.18348628829150834No Hit
TCCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC268650.18213712440701196No Hit
TGAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGC247080.16751327265395316No Hit
TGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGCCACGGCGCACGCGC246380.16703869239307503No Hit
TGCGGCGACACCGGCGCGCCCCCGCGGGGGGAGCCCCCTCGCGGGGGACC243880.16534376288993888No Hit
TGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGC238790.16189288642155367No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA234670.15909964260038525No Hit
TCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC234590.15904540485628488No Hit
TGGCCCATACCCGGCCGTCGCTGGCAGTCGGCAGAGCGAGAAAGAGAGTG234370.1588962510600089No Hit
TGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACC232990.15796064997427775No Hit
TGCGCCGCGCGGAACCGCGGCCCCGGGGGCGGACCCGGCGGGGGGAGACC232760.15780471645998922No Hit
TGGGGAGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGCCACGGCGCA232320.15750640886743728No Hit
TCCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCC225390.15280806428474383No Hit
TCTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCC222350.15074703000893025No Hit
TTGCTGCTGCCGGGGCGGGGCTGTAACACCCGGGGAGGGGTCGCGCCCGC220930.14978431005114892No Hit
TGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGG220000.14915379627598227No Hit
TGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACACCGGCG217810.14766903803123496No Hit
TGGCGACACAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGC217400.14739106959272064No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG215810.14631309442872603No Hit
TGGGGAGAGGGGGGGAGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCG215160.14587241275791063No Hit
TGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA214540.14545207024113288No Hit
TGACAGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACA214060.14512664377653073No Hit
TCCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG211860.1436351058137709No Hit
TCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGGGCTGTAACACCCGG209870.14228594192927455No Hit
TGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGCAAGGCC209710.1421774664410738No Hit
TTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA208720.1415062743578319No Hit
TGGGAGAGGGGGGGAGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGC207680.14080118368452724No Hit
TGACACAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGC207160.14044863834787494No Hit
TGGGGGAGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGCCACGGCGC206600.1400689741391724No Hit
TGGCGCCGCGCGGAACCGCGGCCCCGGGGGCGGACCCGGCGGGGGGAGAC204020.1383198068919359No Hit
TGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGGGCT201880.13686894723725135No Hit
TACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT201500.13661131795277465No Hit
TCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT198340.13446892706081054No Hit
TACACAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCC198110.134312993546522No Hit
TGGAGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGCCACGGCGCACG198110.134312993546522No Hit
TGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGC195800.1327468786856242No Hit
TGGGAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGC194640.13196043139616903No Hit
TCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCG194060.13156720775144143No Hit
TCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTT191090.12955363150171567No Hit
TCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGG190890.12941803714146477No Hit
TGCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA190530.12917396729301317No Hit
TCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC187360.12702479668303654No Hit
TGGAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCG182050.12342476641837531No Hit
TGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCA179070.121404410450637No Hit
TTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT175320.11886201619593277No Hit
TGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACACC174660.11841455480710482No Hit
TACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT173920.1179128556741765No Hit
TGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGG173080.11734335936112276No Hit
TGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACACCGGCGCGC170880.11585182139836295No Hit
TGCGCGGAACCGCGGCCCCGGGGGCGGACCCGGCGGGGGGAGACCGCGCG170560.11563487042196151No Hit
TTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG169800.1151196118530081No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCCGTAAGCACCTC169460.11488910144058159No Hit
TCCCGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC168630.11432638484554039No Hit
TGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGG168140.11399417866292572No Hit
TGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGA168110.11397383950888808No Hit
TGGGGGGGTGGCCGGGGACCCCCGCGGGGGGAGTCCCGCGGCGCGCTCCA166310.11275349026663004No Hit
TCCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA165900.11247552182811571No Hit
TCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTG162500.11017041770385051No Hit
TGCCCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA161590.10955346336470896No Hit
TATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC161230.10930939351625735No Hit
TCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACACCGGCGCGCCC160660.10892294958954232No Hit
TGGGAGCGAGACCTGTGTCGCCGCGCTCTCCCCCCTCCCGGCGCTCCCCC160480.10880091466531651No Hit
TGCGACACAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCT159930.10842803017462656No Hit
TGGGGAGAGCGCGGCGACACAGGTCTCGCTCCCTCGGCCCCGGGATTCGG158800.107661922039209No Hit
TGGAGAGGGGGGGAGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGCC158480.10744497106280758No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCCGTAAGCAGCTC156280.10595343310004775No Hit
TCAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCGGG153620.10415002810871088No Hit
TCGCGCGGAACCGCGGCCCCGGGGGCGGACCCGGCGGGGGGAGACCGCGC150370.10194661975463387No Hit
TCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGGACCGACGGGG149320.10123474936331667No Hit
TTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGCAAGGCCG148590.10073982994840093No Hit
TCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC148590.10073982994840093No Hit
TACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCC147930.10029236855957296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)