FastQCFastQC Report
Sat 30 Sept 2023
SRR19424370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR19424370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13025261
Total Bases989.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC3723372.8585761160563306No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT3415122.6219205895375146No Hit
TCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA2746032.1082341459414904No Hit
TCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2558291.9640988384033151No Hit
TGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG2528681.9413660885566901No Hit
TGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA2259061.7343683170724946No Hit
TCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1980141.5202305734986807No Hit
TCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1656291.27159831960373No Hit
TCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1610231.236236264286758No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG1582451.2149084766900256No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTTCAAGCGATCTC1572061.206931669161946No Hit
TTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1533631.177427461914199No Hit
TCCCCGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCA1178410.9047112376481361No Hit
TCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA1119050.8591382545040749No Hit
TGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGC1113610.854961754701115No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1095920.8413804529521519No Hit
TCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1011900.7768750276865852No Hit
TGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTT951560.7305496603868437No Hit
TGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC915060.7025271892824259No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA907820.6969687593976044No Hit
TCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG747810.574122852509443No Hit
TCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG648790.49810134322836214No Hit
TGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACAC644500.4948077431999251No Hit
TGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA618730.47502311086127186No Hit
TTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA609270.46776030054215423No Hit
TGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC604830.464351539673562No Hit
TCCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTA578080.4438145231792284No Hit
TGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA538910.4137421891200492No Hit
TGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC533780.40980368838674325No Hit
TGCCGCGCGGAACCGCGGCCCCGGGGGCGGACCCGGCGGGGGGAGACCGC518710.39823386264582333No Hit
TGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG517410.39723580203114545No Hit
TGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGCA512720.3936351064289614No Hit
TGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCC510480.3919153712159779No Hit
TCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGG497740.3821343771921346No Hit
TGGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGG497520.3819654746265737No Hit
TTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT494380.3795547743726594No Hit
TCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGGGCTGTAACACCCGGG445600.34210446915420734No Hit
TGTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA444570.3413136980518087No Hit
TCCCGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCAC432740.3322313464582399No Hit
TGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC425070.3263427888316403No Hit
TGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCG400980.30784795790272457No Hit
TGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT399940.30704950941098225No Hit
TAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCC396460.304377777919383No Hit
TGGGAGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGCCACGGCGCAC394800.3031033312883327No Hit
TCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC386040.2963779382232724No Hit
TGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGGA385140.2956869731823416No Hit
TGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAG379160.2910958943548233No Hit
TGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTAT377170.28956809387543175No Hit
TGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGCAA375550.2883243568017562No Hit
TCACAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCG374660.287641069150169No Hit
TCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGAT374210.2872955866297036No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC367680.2822822513882831No Hit
TGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACA366930.28170644718750737No Hit
TTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC365250.28041664577776987No Hit
TCTTTACTAACACCAACTCCTAACCCTACTAAAACCCTCTAATCCTCTAT362060.2779675585771372No Hit
TTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT350060.26875469136472585No Hit
TCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG350050.26874701397538214No Hit
TGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTG348990.26793321070495246No Hit
TCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGG330290.25357649263227816No Hit
TACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT326440.25062069773496287No Hit
TGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGC324270.2489547042473851No Hit
TCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGC318770.24473214010836325No Hit
TCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT318000.2441409811289002No Hit
TTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG314240.241254282735678No Hit
TGTCCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGGACCGAC311930.2394808057972888No Hit
TGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGA306350.23519682254351756No Hit
TAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCG305560.23459030878536713No Hit
TCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGGACCGACGGG302620.23233315631832635No Hit
TGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGCAAGGCCGG285720.21935836832751374No Hit
TGGCGGCGACACCGGCGCGCCCCCGCGGGGGGAGCCCCCTCGCGGGGGAC277830.21330090813535327No Hit
TAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTT277540.21307826384438666No Hit
TCTGCTGCTGCCGGGGCGGGGCTGTAACACCCGGGGAGGGGTCGCGCCCG271950.20878660320127174No Hit
TGCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT259810.19946625253804895No Hit
TCGGGCCCATACCCGGCCGTCGCTGGCAGTCGGCAGAGCGAGAAAGAGAG247570.1900691279813894No Hit
TGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC247270.1898388063010791No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCTCTTATACACA245510.1884875857765921No Hit
TGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGC237610.182422448195088No Hit
TGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACACCGGCGCGCC237110.1820385787279042No Hit
TAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG233440.17922097683877505No Hit
TGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGC227070.17433047982685337No Hit
TCCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC218080.16742850680688856No Hit
TCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC214070.16434987368007442No Hit
TCCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCC209240.1606416946270789No Hit
TGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACC206590.1586071864510047No Hit
TCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGGGCTGTAACACCCGGGG205150.15750164238551534No Hit
TGGCCCATACCCGGCCGTCGCTGGCAGTCGGCAGAGCGAGAAAGAGAGTG203420.15617345402905938No Hit
TCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC203040.15588171323399969No Hit
TGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAG201000.15431552580788976No Hit
TCCGCGCGGAACCGCGGCCCCGGGGGCGGACCCGGCGGGGGGAGACCGCG198070.15206605073019266No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG194860.14960160875087264No Hit
TGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACACCGG194220.14911025583287738No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA192740.1479740022100133No Hit
TGACAGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACA191810.1472600050010514No Hit
TCTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCC190980.1466227816855263No Hit
TCCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG188120.14442704833323494No Hit
TCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCC186690.14332918165708924No Hit
TTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA183450.1408417075097382No Hit
TGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGG182890.14041177370649233No Hit
TGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA181080.13902216623528696No Hit
TCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTT175460.134707473424141No Hit
TGCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA175390.13465373169873524No Hit
TACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT174180.13372476758815044No Hit
TGCCTCACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGG173460.13317199555540576No Hit
TGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCGCAAGGCC171690.1318130976415751No Hit
TGAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGC170140.1306231022933053No Hit
TGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGC170010.1305232962318375No Hit
TGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGC169250.1299398146417181No Hit
TCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGC168790.129586654731909No Hit
TGCGGCGACACCGGCGCGCCCCCGCGGGGGGAGCCCCCTCGCGGGGGACC168280.12919510787538152No Hit
TCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCCCG168030.12900317314178963No Hit
TCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT161470.1239668057323381No Hit
TGGGGGGGTGGCCGGGGACCCCCGCGGGGGGAGTCCCGCGGCGCGCTCCA158090.12137184813417559No Hit
TACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT156930.12048127097030917No Hit
TGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGG156280.11998224066297021No Hit
TTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT156060.11981333809740934No Hit
TTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTT153750.11803986115902015No Hit
TTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG152970.11744102479021343No Hit
TCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGGGCTGTAACACCCGG151910.11662722151978375No Hit
TGGCGACACAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGC151200.11608212687638274No Hit
TATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC150190.11530671055267146No Hit
TCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC149880.11506871148301749No Hit
TGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCA147750.11343342755281449No Hit
TGACACAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGC147640.11334897627003405No Hit
TGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGCCACGGCGCACGCGC147630.11334129888069039No Hit
TTGCTGCTGCCGGGGCGGGGCTGTAACACCCGGGGAGGGGTCGCGCCCGC146480.11245839910616763No Hit
TCCGGAGCGGGTCGCGCCCGGCCGCCGCCGGCCCGCGCGGACCGACGGGG144910.11125304897921047No Hit
TGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGGGCT144680.11107646902430593No Hit
TACCGGGTCAGTGAAAAAACGATAAGAGTAGTGGTATTTCACCGGCGGCC144600.1110150499095565No Hit
TCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCCGGGGCGGG142010.1090266060695444No Hit
TCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTG141770.10884234872529618No Hit
TGGCGGCCCGGCCCCCCCGTGCCGCGCCCGCGAGGGCGGCGACACCGGCG141740.10881931655726514No Hit
TCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCC141080.10831260886058251No Hit
TGGGGAGGGAGACGGGGCTCGGCCGCGCGCGCGGCGCGCGCCACGGCGCA141070.10830493147123885No Hit
TACACAGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGCTGCTGCTGCC140320.10772912727046315No Hit
TCCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA138740.10651609975416232No Hit
TCCCGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC137230.10535681396326721No Hit
TGGGAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCCCGTGCCGC131980.10132618455783726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)