FastQCFastQC Report
Fri 30 Jun 2023
SRR1944912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28905052
Total Bases855 Mbp
Sequences flagged as poor quality0
Sequence length25-49
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA19720606.8225443773635135No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG18611686.438902099190134No Hit
CAAGAGGTGCACAATCGACCGATCCTGA16066735.558450474332307No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3548701.2277092599591242No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC2883050.9974207968904535No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1984000.6863852035277432No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG1976570.6838147186173544No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1685120.5829845938350153No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1560600.5399056192668327No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1210640.4188333582655378No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1161940.40198509243297675No Hit
CAATATAGATGGATACGAATAAGGCGTCC1058370.3661539858153516No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC937200.32423397819869No Hit
AAAGAGGTGCACAATCGACCGATCCTGA886080.3065484884787614No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA800880.2770726722788805No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG697700.24137649017202945No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT652310.2256733528796281No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC619900.21446078007401614No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG596060.20621308690259404No Hit
CAATATAGATGGATACGAATAAGGCGTC589820.20405429473020842No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG566830.1961006678002171No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT487140.16853109276537542No Hit
TAAGAGGTGCACAATCGACCGATCCTGA465500.1610445122188329No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGC420830.14559046633093758No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG405620.1403284104107476No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC403840.1397126011051632No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAT356240.12324489158504195No Hit
TCAATATAGATGGATACGAATAAGGCGTCC352730.12203057098807502No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA347480.12021427949688518No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT339530.1174638952387977No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC320420.11085259421086667No Hit
AATATAGATGGATACGAATAAGGCGTCC315380.10910895437932441No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT297380.10288166926667353No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT293630.1015843182015379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)