FastQCFastQC Report
Fri 30 Jun 2023
SRR1944913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37334344
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length25-49
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA458371512.277475666908732No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG30726758.230156662187502No Hit
CAAGAGGTGCACAATCGACCGATCCTGA14008473.7521671734743753No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG5232731.4015861642031262No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC3441300.9217518325753895No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG2628230.7039711210675083No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2220230.594688365222113No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1866570.49996057249593023No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1823560.4884403486505615No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1548620.41479769940513755No Hit
CAATATAGATGGATACGAATAAGGCGTCC1224370.3279473719961438No Hit
CAATATAGATGGATACGAATAAGGCGTC1168750.3130495610154554No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1011790.2710078420019915No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1009130.2702953612898622No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT939680.251693186305885No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC929330.24892093992598344No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC914710.2450049745081901No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA854170.22878934205995421No Hit
TAAGAGGTGCACAATCGACCGATCCTGA747220.20014279613430463No Hit
AGAGGTGCACAATCGACCGATCCTGA713350.19107072029978617No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT651670.17454973897492346No Hit
CAAGAGGTGCACAATCGACCGATCCTG593680.1590171237507213No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG587870.15746091587949154No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG562550.15067895661967437No Hit
AGTTTCTTTACTTATTCAATGAAGCGGA477540.12790903731963257No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGC460790.12342255163235225No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG458880.12291095833905638No Hit
AATATAGATGGATACGAATAAGGCGTCC455900.12211276566155815No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC444010.11892803044831858No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT406010.10874973456075725No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT404340.1083024252414881No Hit
AAATATAGATGGATACGAATAAGGCGTCC382600.10247936859423591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)