FastQCFastQC Report
Fri 30 Jun 2023
SRR1944914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17505437
Total Bases525.9 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA13158377.516733229795977No Hit
AAGAGGTGCACAATCGACCGATCCTGA4065262.322284213755989No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG3709562.1190902003760317No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2131271.21749031458055No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1643410.9387997568983854No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1483490.8474452822857264No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1202900.6871579384165045No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1089540.6224009146415482No Hit
AACTATGCCGACTAGGGATCGGGTGGTG919110.5250425910532824No Hit
CAATATAGATGGATACGAATAAGGCGTCC828630.47335579226042745No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT590510.33732948226313914No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA565640.3231224676082065No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT536850.3066761486731237No Hit
TCCGATATAGTGTAACGGCTATCACATCACG476470.27218400774570783No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT446860.2552692629152874No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT436160.24915687623222432No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT426100.24341009024796126No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC410580.2345442733020604No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG379610.21685262698668992No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT362330.20698140811908897No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT313300.17897296708445498No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA312880.17873304162586745No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG297710.17006716256212284No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG268870.15359228107244624No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG268230.1532266803736462No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG264640.15117588895381476No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC259070.14799402037207068No Hit
TCAATATAGATGGATACGAATAAGGCGTCC252940.1444922511788766No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGG245660.1403335432300262No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC236680.1352037084249882No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA220760.1261093910423373No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT210930.1204939928092055No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT200280.11441016868073617No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG187650.1071952673903542No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT184810.10557291428942904No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG182400.10419619915801015No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAT178980.10224252042379749No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT176690.10093435542340361No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)