FastQCFastQC Report
Fri 30 Jun 2023
SRR1944915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32467816
Total Bases970 Mbp
Sequences flagged as poor quality0
Sequence length25-49
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAG426224413.127596879321974No Hit
CAAGAGGTGCACAATCGACCGATCCTGA6849962.1097692558070427No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG3363331.0358965937222262No Hit
AAGAGGTGCACAATCGACCGATCCTGA3356631.033833011743075No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC3201600.9860841887239967No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT1557160.47960109173958604No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1473150.4537262376995114No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1151730.3547297422161072No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG1085830.33443271946594744No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC1054740.3248570830880648No Hit
AACTATGCCGACTAGGGATCGGGTGGTG981830.30240099919255425No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT897790.27651690523316996No Hit
CAATATAGATGGATACGAATAAGGCGTCC878610.27060951682121154No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC719110.2214839458249979No Hit
ATGTACACGGAGTCGACCCGCAACGCGA704380.21694714544396826No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT633200.1950238968953132No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG601360.18521726253468973No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGC544030.16755977673398173No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT410200.12634049669371047No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGT377350.11622278504966271No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG368680.11355244836917888No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC347710.10709374477174566No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAA342030.10534432004912188No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCA338730.10432792892506229No Hit

[OK]Adapter Content

Adapter graph