FastQCFastQC Report
Fri 30 Jun 2023
SRR1944916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27617546
Total Bases834.3 Mbp
Sequences flagged as poor quality0
Sequence length25-49
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAG22288688.070478093890022No Hit
CAAGAGGTGCACAATCGACCGATCCTGA8276842.9969498376141024No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC3547201.2844008660291542No Hit
AAGAGGTGCACAATCGACCGATCCTGA3135811.1354412155229143No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG2017260.7304269539371818No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1315910.4764760779252436No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1290350.4672210919826113No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1124180.40705282069594456No Hit
AACTATGCCGACTAGGGATCGGGTGGTG954670.34567517331192277No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT884110.3201261980336703No Hit
CAATATAGATGGATACGAATAAGGCGTCC872930.3160780469053985No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT807470.29237572375184967No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC606130.2194727945777659No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT547320.1981783609593698No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC540570.1957342625590268No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC447690.16210346857030672No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG430130.1557451918428958No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG379300.13734022566668305No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT372720.1349576823371635No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT371890.13465714875608426No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC365590.13237599024909744No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG362570.13128248252035138No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG354180.12824455873088797No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT352750.12772677195866713No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGC340970.1234613676392537No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA333950.12091950530289694No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA320190.11593716545271618No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG310130.11229455361457533No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT302020.10935801464764465No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAT298810.10819571007503709No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT278180.10072582118628498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)