FastQCFastQC Report
Fri 30 Jun 2023
SRR1944917.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944917.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23857016
Total Bases730.2 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA8069063.3822587032678353No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTC5516252.312212893682932No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC3390951.4213638453358963No Hit
TAATGACCCACTCGGCACCTTACGAGAAATC2164940.9074647055608296No Hit
AATGACCCACTCGGCACCTTACGAGAAATC2163720.9069533255961265No Hit
TAGACACCACAAAAGGTGTTAGTTCATCTAGAC1938880.8127085130847882No Hit
AGACACCACAAAAGGTGTTAGTTCATCTAGAC1935660.8113588053090965No Hit
TTCGGGGCTGATCCGGGTTGAAGACATTGTC1877440.7869550827312184No Hit
AACGAGGAATTCCTAGTAAGCGCAAGTCATC1779790.7460237273597No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG1415780.5934438741207199No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1387550.5816108770686158No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGT1276060.5348782932450563No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1203220.5043463943688515No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGC1168850.4899397309370124No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1145430.48012291227033593No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1142610.47894087005684194No Hit
AAGTCTGGTGCCAGCAGCCGCGGTAATTCC1101910.461880899103224No Hit
ACCTTGACCTATTCTCAAACTTTAAATATGT1093230.45824255640353345No Hit
AGAGTTAAGGTGCCGGAATACACGCTCATC1086860.4555724823255347No Hit
AAGAGGTGCACAATCGACCGATCCTGA1060110.44435984785356225No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTG967620.40559137823439445No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGC767890.32187177139001794No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGC759380.3183046865542614No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT709940.29758122306662327No Hit
AAATGACCCACTCGGCACCTTACGAGAAATC692780.2903883704483411No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAT692720.29036322061401143No Hit
AACACGGGGAAACTCACCAGGTCCAGACAC690290.28934465232366025No Hit
TCCGATATAGTGTAACGGCTATCACATCACG679480.2848134905052669No Hit
TAGAGTTAAGGTGCCGGAATACACGCTCATC646790.27111102243465823No Hit
TGGACTTTTACTTTGAAAAAATTAGAGTGTTC646030.27079245786648254No Hit
TGCAGGCCACCATCGAAAGGGAATCCGGTT644600.2701930534816257No Hit
CGGGGCTGATCCGGGTTGAAGACATTGTC641210.268772087842No Hit
AAATGCCTCGTCATCTAATTAGTGACGCGC573950.2405791235584534No Hit
AGAATCCATGCTAGAACGCGGTGATTTC559140.23437130611808285No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCT544510.22823893818070123No Hit
AACTATGCCGACTAGGGATCGGGTGGTG536610.22492754332729625No Hit
AGTTTTCATGCCAGGTCGTACTGATAACCGC534430.22401376601331868No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGC525670.2203418902011886No Hit
ATAGCTGTTGGGACCCGAAAGATGGTGAACT525280.22017841627804585No Hit
ATGGAATAATAGAATAGGACGTTTGGTTCTA509220.21344664395580737No Hit
GCAGGCCACCATCGAAAGGGAATCCGGTT507740.21282628137567583No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC502530.2106424374280505No Hit
CAATATAGATGGATACGAATAAGGCGTCC484750.20318970318836185No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT474570.19892261463042987No Hit
ACTTGCGTGCCTTGTTGTAGACGGCCTTGG466860.19569086091906887No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAT462130.1937082156460808No Hit
TTTGATGATTCATAATAACTTTTCGAATCGC443490.18589500044766705No Hit
ATGTACACGGAGTCGACCCGCAACGCGA425920.1785302906281322No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGC425000.17814465983507743No Hit
TAAACTTTCAACAACGGATCTCTTGGTTCTCGC419890.1760027322780016No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC409760.1717566019153443No Hit
TGAGCGGAGAATTTGGACAAACTTGGTCATTT389400.16322242479948038No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTT369380.1548307634114845No Hit
ACGGGGAAACTCACCAGGTCCAGACACAAT367250.15393794429278163No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCTT366520.1536319546417708No Hit
ATTGCGATGGTCAGAAAGTGATGTTGACGC362120.15178763345759588No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATC359360.1506307410784316No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA356080.14925588346841032No Hit
GACTTTTACTTTGAAAAAATTAGAGTGTTC352520.14776365996485058No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT344410.14436424069129183No Hit
TATACAGTGAAACTGCGAATGGCTCATTAAATC338120.14172769972573268No Hit
AACGGGTAACGGGGAATAAGGGTTCGATTC335400.1405875739027882No Hit
ATCTACCAGAAAGCACCGTTTCCCGTCCGATC331220.13883546877782202No Hit
AGCAGGCGCGCAAATTACCCAATCCTAATTC328470.13768276803771268No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGGG327040.13708336365285584No Hit
CCTTGACCTATTCTCAAACTTTAAATATGT315690.13232585332549554No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGC310270.13005398495771642No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC307540.12890966749571697No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT306460.12845697047778312No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGC302450.126776123216751No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT295230.12374975981908215No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA287360.120450939882842No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC284410.11921440636163383No Hit
GTAGAGTTAAGGTGCCGGAATACACGCTCATC283930.11901320768699657No Hit
ACCCCGGAATTGGTTTATCCGGAGATGGGGTC277690.11639762491671213No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGT273340.11457426192781192No Hit
TTGCTCCACACAATATAGATGGATACGAAT272450.11420120605192201No Hit
ACAATATAGATGGATACGAATAAGGCGTC264740.11096945234056095No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGT253940.10644248216122251No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG252540.10585565269353048No Hit
TTATACAGTGAAACTGCGAATGGCTCATT252050.1056502623798383No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACG249820.104715526870586No Hit
AAGGTCCCATTCGGGGCTGATCCGGGTTGAAGAC243340.10199934476298293No Hit
AGGTCGTACTGATAACCGCAGCAGGTCTCC241790.10134964070946677No Hit
AGAACTGGCGATGCGGGATGAACCGAACGT240360.10075023632460992No Hit
ACTTGCGTGCCTTGTTGTAGACGGCCTTGGT239870.1005448460109177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)