FastQCFastQC Report
Fri 30 Jun 2023
SRR1944918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22548590
Total Bases702.6 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA2092350.9279294182030894No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG1834160.8134255844822226No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1687660.7484547814297923No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1448740.6424969366155489No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1177970.522414040079668No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT921900.40885039818454283No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT834160.36993887422672544No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT786760.3489176041606149No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC786440.3487756884133332No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT713700.31651646510934833No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA575930.2554173010374485No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA525740.23315870304972505No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC497130.22047054826931528No Hit
CAATATAGATGGATACGAATAAGGCGTCC474490.21043000914913085No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT473100.2098135626218757No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT400480.17760755772312148No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC398050.17652988501720063No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC393600.17455636915656367No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG386810.17154509439392884No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG368640.16348694086858645No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT363240.16109211263320677No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT333870.1480669079530028No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT326040.1445944070117023No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT313120.13886455871520126No Hit
AAGAGGTGCACAATCGACCGATCCTGA307730.1364741653469241No Hit
AACTATGCCGACTAGGGATCGGGTGGTG303220.13447404028367185No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA297660.13200825417465126No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA275630.12223824194772268No Hit
ATGTACACGGAGTCGACCCGCAACGCGA262490.11641082657496543No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA259950.11528437033091647No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGA248320.11012661989064504No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG243360.10792692580777778No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG237970.10553653243950065No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC230230.1021039453021231No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGC228730.10143871523673985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)