FastQCFastQC Report
Fri 30 Jun 2023
SRR1944919.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944919.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20240822
Total Bases616 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA7984793.9448941352283025No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2961791.4632755527418797No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2263131.118101824125522No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1984540.9804641333242295No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1554320.7679134770317134No Hit
AAGAGGTGCACAATCGACCGATCCTGA1489180.735730989581352No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1413610.6983955493507131No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1373350.678505052808626No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1077990.5325821253702049No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT971790.4801139005125385No Hit
CAATATAGATGGATACGAATAAGGCGTCC815200.4027504416569644No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC812640.4014856708882673No Hit
AACTATGCCGACTAGGGATCGGGTGGTG643360.3178527038081754No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA598240.29556111900989No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG554050.2737290017174204No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT548220.27084868391214545No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT532570.26311678448632175No Hit
ATGTACACGGAGTCGACCCGCAACGCGA526760.2602463477026773No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT518900.25636310620191216No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG472660.23351818419232181No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA399990.1976154920980976No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA350950.1733872270602449No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT345520.17070452968757888No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT344320.17011166838975217No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT338020.1669991465761618No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC337000.16649521447300905No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT321990.1590795077393596No Hit
TCAATATAGATGGATACGAATAAGGCGTCC309130.15272601083098303No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG293380.1449447062970071No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTC289400.14297838299254842No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT274700.13571583209417087No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT273010.13488088576639823No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT263380.13012317385133865No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT255470.1262152297964974No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC249740.12338431709937472No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG248380.12271240762850441No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG245140.12111168212437223No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG239430.11829065044888No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGC234700.1159537888332796No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT228520.1129005531494719No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAT227810.11254977688159107No Hit
CCGATATAGTGTAACGGCTATCACATCACG217450.10743140767702022No Hit
CTAGAGGTGCACAATCGACCGATCCTGA215570.10650259164375835No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA210610.10405209827940781No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA209910.10370626252234223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)