FastQCFastQC Report
Fri 30 Jun 2023
SRR1944920.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944920.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17539519
Total Bases548.3 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATAGTGTAACGGCTATCACATCACG2036511.161097975377774No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1585410.9039073420428463No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1351400.77048863198586No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC1165800.6646704507689178No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC907120.5171863606978048No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG891560.5083149657638844No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT849520.4843462354925469No Hit
CAAGAGGTGCACAATCGACCGATCCTGA843070.48066882563883306No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC789540.45014917455832176No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT689180.39292981751666056No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT614760.35049991963861726No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG566860.3231901627404948No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA565480.3224033680741188No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG561160.31994035868372445No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC495190.2823281527845775No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG455990.25997862313099923No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA426700.24327919140770052No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT373860.21315293766037713No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA367650.20961236166168523No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA357880.20404208348016842No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC344910.19664735389835947No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT338400.19293573558089022No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA336270.1917213351175708No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC335980.1915559942094193No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT329560.18789568858758326No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTG317050.1807632238945663No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGA291830.16638426629601416No Hit
CAATATAGATGGATACGAATAAGGCGTCC289550.16508434467330604No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG274850.15670327105321416No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA274090.1562699638456448No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT270990.1545025265516118No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG235620.13433663716775815No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT234920.13393753842394424No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG225870.12877776180749312No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT220840.12590995226265897No Hit
AGTCAGAATCCATGCTAGAACGCGGTGATTT210300.11990066546294685No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT201140.1146781733296107No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT200240.11416504637327854No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG193240.11017405893513955No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTGA192430.1097122446744406No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGC190960.10887413731243142No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA182850.10425029329481612No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA179490.10233461932450941No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT176680.10073252293862792No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT175750.100202291750418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)