FastQCFastQC Report
Fri 30 Jun 2023
SRR1944921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29349129
Total Bases895 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA15999455.451422425517296No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG4494501.531391272292953No Hit
AAGAGGTGCACAATCGACCGATCCTGA4175321.4226384708043636No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2967861.0112259208782652No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC2536710.8643220723858619No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC2070320.7054110532547662No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1986290.6767798799071687No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1520450.5180562598637936No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1164500.39677497754703384No Hit
CAATATAGATGGATACGAATAAGGCGTCC1149270.3915857264452379No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT835710.28474780290754115No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC797210.27162986676708534No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA752660.25645054066170075No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT713190.24300210067562822No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA698850.238116095370326No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC674630.22986372099833013No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT662610.2257681991175956No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG588430.20049317306827058No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT581950.1982852710893056No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT579950.19760381986122996No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT555730.1893514454892341No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC547660.18660178978394895No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT525680.1791126407873978No Hit
CTAGAGGTGCACAATCGACCGATCCTGA496880.1692997431031088No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT483680.16480216499780964No Hit
AACTATGCCGACTAGGGATCGGGTGGTG460500.15690414526441313No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT458290.15615114165738955No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC437030.14890731510294564No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT412550.14056635207129997No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG386370.13164615549579No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT385760.13143831287122695No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT382930.13047405938349993No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGG372320.12685896061855872No Hit
ATCTTTGAACGCACATTGCGCCCCTTGGT361010.12300535392379107No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG353680.12050783517289387No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG351750.11985023473780092No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA340280.11594211194478718No Hit
TCAATATAGATGGATACGAATAAGGCGTCC333710.11370354466055876No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGC321380.10950239783947252No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG317360.1081326808710405No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT294300.10027554821132852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)