FastQCFastQC Report
Fri 30 Jun 2023
SRR1944922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26332332
Total Bases807.5 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA9951883.779338647256916No Hit
AAGAGGTGCACAATCGACCGATCCTGA3400501.2913782189894916No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC2724121.0345152871382604No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1897370.720547652217054No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1774300.6738104319814895No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1663190.6316151566067145No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG1370510.5204666263512095No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1366180.5188222600261914No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1327520.5041406890965829No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1160580.4407433416835243No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1023880.38882997525627433No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC975640.37051029130272245No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC950250.36086815250544463No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT928610.35265011849311334No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA837030.3178715808383398No Hit
AACTATGCCGACTAGGGATCGGGTGGTG835740.31738168879231815No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT816070.30991178449367873No Hit
CAATATAGATGGATACGAATAAGGCGTCC792360.300907644640057No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT774050.29395421567675817No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA642730.24408396491431145No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC632070.2400357097123035No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT534680.20305075904405276No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT499280.18960720987415774No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT495280.1880881647702148No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT441540.16767979379874143No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT434210.16489614364576596No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT416670.15823513086497618No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT396740.1506664886345805No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA384480.14601061539099536No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG381350.14482196259716001No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC356100.1352329903785202No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA353540.13426080151199674No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGC338720.12863273940188813No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG338190.1284314659256157No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC329900.12528324494769397No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG324900.12338443856776528No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT313060.11888806506009418No Hit
ATCTTTGAACGCACATTGCGCCCCTTGGT304920.11579680827357029No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG304630.11568667750353444No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT296560.11262200400632956No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA295890.11236756395141911No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGG284290.10796233314998459No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG282990.10746864349120314No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG281280.10681925170926752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)